Document Detail


Influence of ultralow temperatures on the translating activity of cell-free extracts of different origins.
MedLine Citation:
PMID:  550905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of various systems of freezing (-196 degrees C) and thawing on the protein-synthesizing activity of cell-free extracts from Krebs II ascites carcinoma of the rat liver and from wheat germ was studied. It was established that the biosynthesis of proteins, stimulated by exogenous templates, is decreased in the case of slow systems of freezing and warming of cell-free extracts from Krebs II ascites carcinoma. Similar results were obtained in an investigation of the endogenous protein-synthesizing activity of a cell-free extract from the rat liver and Krebs II ascites carcinoma. No significant decrease in the translating activity of the cell-free extract from wheat germ was observed in the investigated systems of freezing and warming. It was shown that the greatest sensitivity to the action of low temperatures is possessed by a cell-free extract from Krebs II ascites carcinoma.
Authors:
A K Gulevskii
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biology bulletin of the Academy of Sciences of the USSR     Volume:  6     ISSN:  0098-2164     ISO Abbreviation:  Biol Bull Acad Sci USSR     Publication Date:    1979 May-Jun
Date Detail:
Created Date:  1980-11-20     Completed Date:  1980-11-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7513560     Medline TA:  Biol Bull Acad Sci USSR     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  362-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Krebs 2 / metabolism
Cell-Free System
Cold Temperature*
Liver Neoplasms / metabolism
Plants / metabolism
Protein Biosynthesis*
Rats
Triticum / metabolism

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