| Influence of tris(4-chlorophenyl)methanol, non-ortho PCB 77 and gamma-hexachlorocyclohexane on human sperm function in vitro. | |
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MedLine Citation:
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PMID: 16529574 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In recent years, there has been growing concern that environmental pollutants in general, and organochlorines in particular, adversely affect male fertility. Therefore, we investigated the effects of tris(4-chlorophenyl)methanol (TCPM), non-ortho PCB 77 and gamma-hexachlorocyclohexane (gamma-HCH, lindane) on human sperm functions in vitro. Human spermatozoa from healthy donors were washed in human tubular fluid medium containing 1% human serum albumin, filtered through glass wool and exposed to different concentrations of TCPM, PCB 77 or gamma-HCH. After incubation for 5 h at 37 degrees C and 5% CO(2), sperm vitality and the percentage of living acrosome-reacted spermatozoa were examined using triple stain technique. Total sperm motility was evaluated by computer-assisted sperm analysis (Stroemberg-Mika) after 5 h. For TCPM, total motility was additionally measured after 18 and 40 h. Different concentrations of PCB 77 and gamma-HCH did not alter the percentage of spontaneous living acrosome-reacted spermatozoa, vitality and total motility. TCPM dose-dependently altered sperm motility, vitality and acrosome reaction. The percentage of living acrosome-reacted spermatozoa was increased at overtly toxic concentrations. Therefore, it is suggested that unspecific acrosomal loss has been induced by degenerative processes. In conclusion, even high concentrations of PCB 77 and gamma-HCH did not affect human sperm functions in vitro. Only very high cytotoxic TCPM concentrations modulated spontaneous acrosome reaction and total motility. Therefore, in vivo effects on human sperm function seem to be unlikely. However, individual susceptibility has to be considered and little is known about additive and possible synergistic effects as other environmental pollutants with similar potencies have been found in the human male and female reproductive tract. |
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Authors:
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S Pflieger-Bruss; S Heitkamp; S Hagemann; W Körner; F-M Köhn; C Müller; W-B Schill |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Andrologia Volume: 38 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-13 Completed Date: 2006-06-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: Germany |
Other Details:
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Languages: eng Pagination: 39-47 Citation Subset: IM |
Affiliation:
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Centre of Dermatology and Andrology, Justus Liebig University, Giessen, Germany. sybille.pflieger-bruss@derma.med.uni-giessen.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome Reaction
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drug effects* Culture Media Endocrine Disruptors / toxicity Humans Lindane / toxicity* Male Polychlorinated Biphenyls / analysis, toxicity* Sperm Motility / drug effects Spermatozoa / drug effects*, physiology Trityl Compounds / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Endocrine Disruptors; 0/Polychlorinated Biphenyls; 0/Trityl Compounds; 3010-80-8/tris(4-chlorophenyl)methanol; 32598-13-3/3,4,3',4'-tetrachlorobiphenyl; 58-89-9/Lindane |
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