Document Detail


Influence of tris(4-chlorophenyl)methanol, non-ortho PCB 77 and gamma-hexachlorocyclohexane on human sperm function in vitro.
MedLine Citation:
PMID:  16529574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, there has been growing concern that environmental pollutants in general, and organochlorines in particular, adversely affect male fertility. Therefore, we investigated the effects of tris(4-chlorophenyl)methanol (TCPM), non-ortho PCB 77 and gamma-hexachlorocyclohexane (gamma-HCH, lindane) on human sperm functions in vitro. Human spermatozoa from healthy donors were washed in human tubular fluid medium containing 1% human serum albumin, filtered through glass wool and exposed to different concentrations of TCPM, PCB 77 or gamma-HCH. After incubation for 5 h at 37 degrees C and 5% CO(2), sperm vitality and the percentage of living acrosome-reacted spermatozoa were examined using triple stain technique. Total sperm motility was evaluated by computer-assisted sperm analysis (Stroemberg-Mika) after 5 h. For TCPM, total motility was additionally measured after 18 and 40 h. Different concentrations of PCB 77 and gamma-HCH did not alter the percentage of spontaneous living acrosome-reacted spermatozoa, vitality and total motility. TCPM dose-dependently altered sperm motility, vitality and acrosome reaction. The percentage of living acrosome-reacted spermatozoa was increased at overtly toxic concentrations. Therefore, it is suggested that unspecific acrosomal loss has been induced by degenerative processes. In conclusion, even high concentrations of PCB 77 and gamma-HCH did not affect human sperm functions in vitro. Only very high cytotoxic TCPM concentrations modulated spontaneous acrosome reaction and total motility. Therefore, in vivo effects on human sperm function seem to be unlikely. However, individual susceptibility has to be considered and little is known about additive and possible synergistic effects as other environmental pollutants with similar potencies have been found in the human male and female reproductive tract.
Authors:
S Pflieger-Bruss; S Heitkamp; S Hagemann; W Körner; F-M Köhn; C Müller; W-B Schill
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Andrologia     Volume:  38     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-13     Completed Date:  2006-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  39-47     Citation Subset:  IM    
Affiliation:
Centre of Dermatology and Andrology, Justus Liebig University, Giessen, Germany. sybille.pflieger-bruss@derma.med.uni-giessen.de
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MeSH Terms
Descriptor/Qualifier:
Acrosome Reaction / drug effects*
Culture Media
Endocrine Disruptors / toxicity
Humans
Lindane / toxicity*
Male
Polychlorinated Biphenyls / analysis,  toxicity*
Sperm Motility / drug effects
Spermatozoa / drug effects*,  physiology
Trityl Compounds / toxicity*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Endocrine Disruptors; 0/Polychlorinated Biphenyls; 0/Trityl Compounds; 3010-80-8/tris(4-chlorophenyl)methanol; 32598-13-3/3,4,3',4'-tetrachlorobiphenyl; 58-89-9/Lindane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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