Document Detail

Influence of transmembrane domains on the fusogenic abilities of human and murine leukemia retrovirus envelopes.
MedLine Citation:
PMID:  7769674     Owner:  NLM     Status:  MEDLINE    
The envelopes of two highly divergent oncoviruses, human T-cell leukemia virus type 1 (HTLV-1) and Friend murine leukemia virus (F-MuLV), have distinct patterns of cellular receptor recognition, fusion, and syncytium formation. To analyze the influence of the transmembrane envelope subunit (TM) on fusogenic properties, we substituted either the entire TM or distinct domains from F-MuLV for the corresponding domains in the HTLV-1 envelope. Parental, chimeric, and truncated envelopes cloned into a eukaryotic expression vector were monitored for fusogenic potential in human, rat, and murine indicator cell lines by using a quantitative assay. This highly sensitive assay allowed us to assess the fusogenic properties and syncytium-forming abilities of the HTLV-1 envelope in murine NIH 3T3 cells. All chimeric envelopes containing extracellular sequences of the F-MuLV TM were blocked in their maturation process. Although deletions of the HTLV-1 cytoplasmic domain, alone and in combination with the membrane-spanning domain, did not prevent envelope cell surface expression, they impaired and suppressed fusogenic properties, respectively. In contrast, envelopes carrying substitutions of membrane-spanning and cytoplasmic domains were highly fusogenic. Our results indicate that these two domains in F-MuLV and HTLV-1 constitute structural entities with similar fusogenic properties. However, in the absence of a cytoplasmic domain, the F-MuLV membrane-spanning domain appeared to confer weaker fusogenic properties than the HTLV-1 membrane-spanning domain.
C Denesvre; P Sonigo; A Corbin; H Ellerbrok; M Sitbon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  69     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-07-06     Completed Date:  1995-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4149-57     Citation Subset:  IM; X    
Institut Cochin de Génétique Moléculaire, CNRS UPR415, Université Paris V, France.
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MeSH Terms
3T3 Cells
Amino Acid Sequence
Base Sequence
Cell Fusion
Friend murine leukemia virus / physiology*
Genes, env
Human T-lymphotropic virus 1 / physiology*
Molecular Sequence Data

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