| Influence of temperature and ontogeny on the levels of glucosinolates in broccoli (Brassica oleracea Var. italica) sprouts and their effect on the induction of mammalian phase 2 enzymes. | |
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MedLine Citation:
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PMID: 12358509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Broccoli inflorescences have been recognized as components of healthy diets on the basis of their high content of fiber, vitamin C, carotenoids, and glucosinolates/isothiocyanates. Broccoli sprouts have been recently shown to have high levels of glucoraphanin (4-methylsulfinylbutyl glucosinolate), the precursor of the chemoprotective isothiocyanate, sulforaphane. This study evaluated the effects of temperature and developmental stage on the glucosinolate content of broccoli sprouts. Seedlings cultivated using a 30/15 degrees C (day/night) temperature regime had significantly higher glucosinolate levels (measured at six consecutive days postemergence) than did sprouts cultivated at lower temperatures (22/15 and 18/12 degrees C; p < 0.001). Both higher (33.1 degrees C) and lower (11.3 degrees C) constant temperatures induced higher glucosinolate levels in sprouts grown to a uniform size. Glucosinolate levels were highest in cotyledons and lowest in roots of sprouts dissected both early and late in the 11 day developmental span investigated. Nongerminated seeds have the highest glucosinolate levels and concordantly greater induction of mammalian phase 2 detoxication enzymes. Levels decline as sprouts germinate and develop, with consistently higher glucosinolate content in younger developmental stages, independent of the temperature regime. Temperature stress or its associated developmental anomalies induce higher glucosinolate levels, specific elevations in glucoraphanin content, and parallel induction of phase 2 chemoprotective enzymes. |
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Authors:
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Fernanda Maria Valente Pereira; Eduardo Rosa; Jed W Fahey; Katherine K Stephenson; Rosa Carvalho; Alfredo Aires |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 50 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-02 Completed Date: 2002-11-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6239-44 Citation Subset: IM |
Affiliation:
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Department of Plant Science and Agricultural Engineering, Universidade de Trás-os-Montes e Alto Douro, Apartado 1013, 5001-911 Vila Real, Portugal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brassica / chemistry*, growth & development Cotyledon / chemistry Enzyme Induction / drug effects* Glucosinolates / analysis*, pharmacology* NAD(P)H Dehydrogenase (Quinone) / biosynthesis Plant Roots / chemistry Seedling / chemistry*, growth & development Temperature* |
| Grant Support | |
ID/Acronym/Agency:
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P01CA44530/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glucosinolates; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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