Document Detail


Influence of a subinhibitory dose of antifungal fatty acids from Sporothrix flocculosa on cellular lipid composition in fungi.
MedLine Citation:
PMID:  8898307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antifungal fatty acids produced by the biocontrol fungus Sporothrix flocculosa were studied on the basis of their effect on growth and cellular lipid composition of three fungi, Cladosporium cucumerinum, Fusarium oxysporum, and S. flocculosa, whose growth was decreased by 51, 33, and 5%, respectively, when exposed to 0.4 mg fatty acid per ml. The sensitivity to fatty acid antibiotics from S. flocculosa was related to a high degree of unsaturation of phospholipid fatty acids and a low proportion of sterols. The major responses of sensitive fungi to sublethal doses of antifungal fatty acids from liquid culture of S. flocculosa were: (i) a decrease in total lipid; (ii) an increase in the degree of fatty acid unsaturation (18:1 > 18:2 > 18:3); (iii) an increase in free fatty acids and phosphatidic acid and a decrease in total phospholipids; and (iv) an increase in sterol/phospholipid ratio. These modifications in lipid composition led to an increase in membrane fluidity in sensitive fungi as demonstrated by assessment of fluoresence anisotropy using liposomes and 1,6-diphenyl-1,3,5-hexatriene probe. This alteration in the physical state of lipids appears to be responsible for the previously demonstrated alteration of membrane structure and function in fungi confronted to S. flocculosa.
Authors:
M Benyagoub; C Willemot; R R Bélanger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  31     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-04     Completed Date:  1997-02-04     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1077-82     Citation Subset:  IM    
Affiliation:
Department of Plant Science, Faculty of Agriculture and Food Science, Laval University, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antifungal Agents / pharmacology*
Fatty Acids / pharmacology*
Fluorescence Polarization
Fusarium / drug effects*,  growth & development,  metabolism
Lipid Metabolism*
Sporothrix / metabolism*
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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