Document Detail


Influence of subculturing on gene expression in a Theileria lestoquardi-infected cell line.
MedLine Citation:
PMID:  19178888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study potential molecular markers for identification of attenuation in a Theileria lestoquardi-infected cell line to be used in vaccination trials were identified. Two markers associated with attenuation in Theileria annulata vaccine strains were analyzed (metalloproteinase activity and TNF? mRNA expression). The result showed a decreased activity of MMP 9 and decreased mRNA expression of TNF? with increasing passage number. Suppression subtractive hybridization was used to identify potential new markers of attenuation. Random screening revealed nine differentially expressed genes, one from the parasite and eight from the host. Quantitative real time-PCR confirmed mRNA expression of the parasite vacuolar H+ATPase to be downregulated at higher passages.
Authors:
Awadia M Ali; Doreen Beyer; Mohammed A Bakheit; Birgit Kullmann; Diaeldin A Salih; Jabbar S Ahmed; Ulrike Seitzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Vaccine     Volume:  26 Suppl 6     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-30     Completed Date:  2009-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  G17-23     Citation Subset:  IM    
Affiliation:
Division of Veterinary Infection Biology and Immunology, Research Center Borstel, Parkallee 22, 23845 Borstel, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Line
Gene Expression Regulation*
Host-Pathogen Interactions
Matrix Metalloproteinase 9 / genetics,  metabolism*
Protozoan Proteins / genetics*,  metabolism
Serial Passage
Sheep
Theileria / growth & development*,  pathogenicity*
Tumor Necrosis Factor-alpha / genetics,  metabolism*
Vacuolar Proton-Translocating ATPases / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Protozoan Proteins; 0/Tumor Necrosis Factor-alpha; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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