| Influence of structurally different lipid emulsions on human neutrophil oxygen radical production. | |
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MedLine Citation:
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PMID: 10231349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The aim of this study was to evaluate immunomodulatory properties of lipid emulsions applied in parenteral nutrition by measuring neutrophil oxygen radical production (the 'respiratory burst') after lipid incubation. MATERIALS AND METHODS: Neutrophils, isolated from the blood of 10 healthy individuals, were incubated in medium or in lipid emulsions in a physiological concentration (2.5 mmol L-1) containing long-chain [Intralipid (LCT)], mixed medium and long-chain [Lipofundin (LCT/MCT)] or structured triglycerides (SL). After washing and stimulation with phorbol 12-myristate 13-acetate (PMA) or serum-treated zymosan (STZ) particles, the respiratory burst was evaluated by measuring maximum oxygen uptake, superoxide and hydrogen peroxide production rates and chemiluminescence. RESULTS: Unlike LCT and SL, LCT/MCT increased PMA- and STZ-induced oxygen uptake rate by 45% and 31% respectively (both P = 0.006 compared with medium) as well as superoxide (+56%, P = 0.006) and hydrogen peroxide (+14%, P = 0.04) production, within 5 min after stimulation. Increased LCT/MCT-mediated early respiratory burst was confirmed by decreased PMA- (-65%) and STZ-stimulated (-54%) chemiluminescence peak time (time after stimulation to peak) in combination with unchanged peak height (maximum rate of radical production). Late respiratory burst (within 2 h) of LCT/MCT, indicated by overall luminescence (overall radical production), remained unchanged (PMA) or decreased (STZ, P = 0.02). The addition of 2.5 mmol L-1 LCT/MCT or MCT emulsion to unstimulated neutrophils, in contrast to LCT and SL, resulted in significant luminescence. CONCLUSIONS: Early neutrophil respiratory burst is accelerated by the LCT/MCT emulsion Lipofundin, whereas LCT (Intralipid) and structured lipid emulsions exert no effect. MCT-containing emulsions, contrary to LCT and structured lipid emulsions, can induce oxygen radical production in unstimulated neutrophils. |
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Authors:
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G J Wanten; A H Naber; J W Kruimel; A T Tool; D Roos; J B Jansen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of clinical investigation Volume: 29 ISSN: 0014-2972 ISO Abbreviation: Eur. J. Clin. Invest. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-05-26 Completed Date: 1999-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0245331 Medline TA: Eur J Clin Invest Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 357-63 Citation Subset: IM |
Affiliation:
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Department of Gastroenterology, Academic Hospital, Nijmegen, The Netherlands. G.Wanten@gastro.azn.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chemiluminescent Measurements Emulsions Female Humans Hydrogen Peroxide / metabolism Indicators and Reagents Lipids / chemistry, pharmacology* Luminol Male Middle Aged Neutrophils / drug effects, metabolism* Respiratory Burst / drug effects, physiology* Superoxides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Emulsions; 0/Indicators and Reagents; 0/Lipids; 11062-77-4/Superoxides; 521-31-3/Luminol; 7722-84-1/Hydrogen Peroxide |
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