Document Detail


Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix.
MedLine Citation:
PMID:  20630322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little is understood about the impact of environmental conditions on the virulence plasticity of Listeria monocytogenes strains grown in food. In this report, we monitored changes in the virulence properties of one high virulent (CCUG 3998) and one low virulent (442) L. monocytogenes strains grown on raw salmon (Salmo salar L.). The effect of temperature exposures (0 degrees C, 4 degrees C and 20 degrees C) on the expression levels of virulence genes (hlyA, actA, inlA and prfA), invasion into Caco-2 cells and in vivo mouse infection was analysed. Our results showed that L. monocytogenes virulence genes are differentially expressed when salmon is stored at different temperatures. Of the four virulence genes, the transcript levels for inlA were strongly affected, which correlated with the strain's virulence capacity as assessed by Caco-2 cells. In contrast to CCUG 3998, the virulence of strain 442 was altered with tested conditions. This strain maintains its low virulence status as far as salmon is stored at lower temperatures, but increases its virulence at higher temperatures. These results lead to the indication that exposure to abuse temperature conditions might influence the virulence potential of low pathogenic L. monocytogenes strains in salmon.
Authors:
Samuel Duodu; Arne Holst-Jensen; Taran Skjerdal; Jean-Michel Cappelier; Marie-France Pilet; Semir Loncarevic
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-29
Journal Detail:
Title:  Food microbiology     Volume:  27     ISSN:  1095-9998     ISO Abbreviation:  Food Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-10-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  795-801     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Affiliation:
National Veterinary Institute, Ullevaalsveien 68, Sentrum N-0106, Oslo, Norway. samuel.duodu@vetinst.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion / physiology
Caco-2 Cells
Colony Count, Microbial
Consumer Product Safety
Food Microbiology
Food Preservation / methods*
Gene Expression Regulation, Bacterial / genetics
Humans
Listeria monocytogenes / genetics,  pathogenicity*
Salmon / microbiology*
Seafood / microbiology*
Temperature
Virulence / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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