| Influence of storage on monodispersed cells of Mycobacterium terrae used for quantitative carrier test prEN 14563. | |
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MedLine Citation:
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PMID: 14602657 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The degree of cell clumping increased with time of storage (1% cell clumps immediately after homogenization and 3 and 6.5% after 48 and 96 h of storage, respectively), and the number of living single cells decreased. Quantitative carrier tests were carried out with these cells using ortho-phthaldialdehyde (OPA) and coco fatty aminoxethylate as biocides. In contrast to OPA, with coco fatty aminoxethylate the reductions obtained with freshly homogenized mycobacteria were significantly higher (P = 0.02) than those obtained with mycobacteria kept in the refrigerator for 4 days. Therefore, it is advisable to prepare the test suspension freshly for each test. |
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Authors:
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Eva Woelk; Peter Goroncy-Bermes; Wolfgang Sand |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 69 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-11-06 Completed Date: 2004-02-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6932-4 Citation Subset: IM |
Affiliation:
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Institute of Botany, Department of Microbiology, University of Hamburg, D-22602 Hamburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Colony Count, Microbial Disinfectants / pharmacology* Equipment Contamination Europe Microbial Sensitivity Tests / standards Mycobacteria, Atypical* / cytology, drug effects Specimen Handling / methods* o-Phthalaldehyde / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Disinfectants; 643-79-8/o-Phthalaldehyde |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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