| Influence of sterol structure on phospholipid phase behavior as detected by parinaric acid fluorescence spectroscopy. | |
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MedLine Citation:
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PMID: 3711085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipid-sterol interactions were investigated using parinaric acid fluorescence spectroscopy. Cholesterol and cholesterol analogues which were modified in the sterol nucleus or side chain were added at 50 mol % to multilamellar vesicles of model phospholipids selected to be representative of major components in an LM cell plasma membrane. These included sphingomyelins and saturated and monounsaturated phosphatidylcholines and phosphatidylethanolamines. Based on the changes in cis-parinaric acid steady-state fluorescence polarization observed with addition of sterol, 50 mol % cholesterol abolished the phase transition of all the model phospholipids. Dihydrocholesterol and trans-22-dehydrocholesterol behaved like cholesterol in the two systems studied. 24-Methylcholesterols interacted well with all phospholipids except phosphatidylethanolamine which contained an unsaturated fatty acid. 24-Alkyl,trans-22-dehydrocholesterols abolished the phase transition in only two systems: sphingomyelins and phosphatidylcholines possessing relatively short saturated acyl chains. Since steady-state anisotropy is a function of fluorescence lifetime, rotational diffusion rates, and limiting anisotropy, we determined these parameters for two of the phospholipid systems. The results show that steady-state anisotropy values for phospholipid-sterol interactions correlate closely with limiting anisotropy and to a lesser extent with rotational relaxation time. The behavior of the sterols in the model phospholipids are used to interpret 1) fluorescence polarization measurements made with phospholipids extracted from LM cell plasma membranes, and 2) changes in membrane lipid composition which accompany growth of LM cells on various sterols. |
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Authors:
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C Rujanavech; P A Henderson; D F Silbert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 261 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1986 Jun |
Date Detail:
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Created Date: 1986-07-02 Completed Date: 1986-07-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7204-14 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cholesterol / pharmacology Fatty Acids / analysis Fatty Acids, Unsaturated / diagnostic use* Fibroblasts / analysis, cytology Fluorescence Polarization Growth Substances / pharmacology Membrane Lipids / analysis Mice Phosphatidylcholines Phospholipids* / analysis Sphingomyelins Sterols / pharmacology* Structure-Activity Relationship |
| Grant Support | |
ID/Acronym/Agency:
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GM 30244/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Growth Substances; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phospholipids; 0/Sphingomyelins; 0/Sterols; 18427-44-6/parinaric acid; 4539-70-2/1,2-distearoyllecithin; 57-88-5/Cholesterol |
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