| Influence of spray-dried hydroxyapatite-5-fluorouracil granules on cell lines derived from tissues of mesenchymal origin. | |
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MedLine Citation:
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PMID: 18978702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS. |
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Authors:
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Tim Scharnweber; Catarina Santos; Ralf-Peter Franke; Maria Margarida Almeida; Maria Elisabete V Costa |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-01 |
Journal Detail:
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Title: Molecules (Basel, Switzerland) Volume: 13 ISSN: 1420-3049 ISO Abbreviation: Molecules Publication Date: 2008 |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2009-01-15 Revised Date: 2009-10-27 |
Medline Journal Info:
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Nlm Unique ID: 100964009 Medline TA: Molecules Country: Switzerland |
Other Details:
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Languages: eng Pagination: 2729-39 Citation Subset: IM |
Affiliation:
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Institute for Biological Interfaces; Forschungszentrum Karlsruhe GmbH, 76344 Eggenstein- Leopoldshafen Karlsruhe, Germany. tim.scharnweber@ibg.fzk.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antimetabolites, Antineoplastic / chemistry, pharmacology Cell Line Cell Line, Tumor Cell Lineage Cell Proliferation / drug effects* Dose-Response Relationship, Drug Durapatite / chemistry* Fibroblasts / cytology, drug effects Fluorouracil / chemistry, pharmacology* Mesoderm / cytology Mice Microscopy, Electron, Scanning Microspheres Osteosarcoma / pathology Rats |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites, Antineoplastic; 1306-06-5/Durapatite; 51-21-8/Fluorouracil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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