| Influence of some physical factors on survival of Marek's disease vaccine virus. | |
| | |
MedLine Citation:
|
PMID: 987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell-associated Marek's disease (MD) vaccine was suspended at dilutions normally used for vaccination in seven commercially available diluents and in tryptose phosphate broth. The stability of diluted vaccines was determined by assay in cell cultures subjected to 0 to 37 C for 0 to 90 minutes. Optimum holding temperatures for MD vaccine virus survival varied with the specific diluents employed. Some diluents afforded greatest survival when dilution was at 0 C and held at 0 C, while others performed best when dilution was at 25 C followed by cooling and holding at 0 C. Diluents which allowed greatest survival when tested at 37 C also performed well under other temperature regimes. Spectinomycin dihydrochloride pentahydrate and various buffering compounds were added to commercial diluents used for diluting MD vaccine. Additives producing osmolality of 745 mOsm/kg and higher markedly reduced vaccine virus survival. The adverse effects of high osmotic pressure were accentuated by extended holding time, elevated incubation temperature, and physical manipulations including mechanical mixing or expressing through a syringe and needle. Satisfactory MD vaccine virus survival was afforded by a commercial diluent especially formulated to accommodate the pH osmolality changes produced by adding spectinomycin dihydrochloride pentahydrate. |
| | |
Authors:
|
W M Colwell; D G Simmons; J R Harris; T G Fulp; J H Carrozza; T A Maag |
Related Documents
:
|
8853997 - Vaccine transport and storage: environmental challenges. 15030227 - Furfuryl ethyl ether: important aging flavor and a new marker for the storage condition... 677877 - Transduction in bacillus thuringiensis. 1471417 - The cold chain from manufacturer to vaccinator: experiments and experiences. 18547687 - Evaluation of iscomatrix and iscom vaccines for immunisation against helicobacter pylori. 16968127 - Live, attenuated influenza a h5n1 candidate vaccines provide broad cross-protection in ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Avian diseases Volume: 19 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 1975 Oct-Dec |
Date Detail:
|
Created Date: 1976-02-21 Completed Date: 1976-02-21 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 781-90 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Herpesvirus 2, Gallid
/
drug effects,
growth & development,
immunology* Hydrogen-Ion Concentration Osmotic Pressure Spectinomycin / pharmacology Temperature Viral Vaccines* |
| Chemical | |
Reg. No./Substance:
|
0/Viral Vaccines; 1695-77-8/Spectinomycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in the acid base status of sheep anaesthetised with a combination of atropine sulphate acepr...
Next Document: Molecular nature of beta-galactosidase from different tissues in two strains of the house mouse.