Document Detail

Influence of root-surface conditioning with acid and chelating agents on clot stabilization.
MedLine Citation:
PMID:  20305869     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare the adhesion and maturation of blood components on chemically conditioned root surfaces.
METHOD AND MATERIALS: Clinical root samples of human teeth were obtained (n = 150) and manually scaled. Five groups of 30 samples were treated as follows: (1) saline solution irrigation (control); (2) 24% EDTA gel; (3) 25% citric acid solution; (4) tetracycline solution (50 mg/mL); and (5) 30% sodium citrate solution. After these treatments, 15 samples of each group received a blood drop and were analyzed by SEM. The remaining 15 had their surface morphology evaluated for collagen fibrils exposure by SEM. Photomicrographs were analyzed according to the score of adhesion of blood components. Kruskal-Wallis and Dunn multiple comparison tests were employed.
RESULTS: The control group was characterized by the absence of blood elements on the surface. The best result was observed in the citric acid group, which had a dense fibrin network with blood elements adhered. The EDTA group showed a moderate fibrin network formation. In contrast, a scarce fibrin network and a few cells were present in the tetracycline samples, and an absence of blood elements was found on sodium citrate specimens. The citric acid group was statistically different from the control group (P < .01). No differences were found among the control, EDTA, tetracycline, and sodium citrate groups (P > .05).
CONCLUSION: Under these experimental conditions, citric acid is indicated to stabilize clots on the root surface, which act as a scaffold for connective tissue cell development.
Fábio Renato Manzolli Leite; José Eduardo Cezar Sampaio; Daniela Leal Zandim; Andréa Abi Rached Dantas; Elza Regina Manzolli Leite; Amauri Antiquera Leite
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Publication Detail:
Type:  Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Quintessence international (Berlin, Germany : 1985)     Volume:  41     ISSN:  1936-7163     ISO Abbreviation:  Quintessence Int     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-06-10     Revised Date:  2013-04-04    
Medline Journal Info:
Nlm Unique ID:  0342677     Medline TA:  Quintessence Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  341-9     Citation Subset:  D    
Department of Semilogy and Clinics, School of Dentistry, Federal University of Pelotas (UFPel) Pelotas, Rio Grande do Sul, Brazil.
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MeSH Terms
Acid Etching, Dental / methods*
Blood Cells / ultrastructure
Blood Coagulation / drug effects*
Cell Adhesion / drug effects
Chelating Agents / therapeutic use*
Citrates / therapeutic use
Citric Acid / therapeutic use
Collagen / ultrastructure
Dental Cementum / drug effects,  ultrastructure
Dental Scaling
Dentin / drug effects*,  ultrastructure
Edetic Acid / therapeutic use
Fibrin / drug effects,  ultrastructure
Materials Testing
Microscopy, Electron, Scanning
Root Planing
Single-Blind Method
Smear Layer
Sodium Chloride
Tetracycline / therapeutic use
Tooth Root / drug effects*,  ultrastructure
Reg. No./Substance:
0/Chelating Agents; 0/Citrates; 1Q73Q2JULR/sodium citrate; 60-00-4/Edetic Acid; 60-54-8/Tetracycline; 7647-14-5/Sodium Chloride; 77-92-9/Citric Acid; 9001-31-4/Fibrin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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