Document Detail

Influence of quercetin-rich food intake on microRNA expression in lung cancer tissues.
MedLine Citation:
PMID:  23035181     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Epidemiologic studies have reported that frequent consumption of quercetin-rich foods is inversely associated with lung cancer incidence. A quercetin-rich diet might modulate microRNA (miR) expression; however, this mechanism has not been fully examined.
METHODS: miR expression data were measured by a custom-made array in formalin-fixed paraffin-embedded tissue samples from 264 lung cancer cases (144 adenocarcinomas and 120 squamous cell carcinomas). Intake of quercetin-rich foods was derived from a food-frequency questionnaire. In individual-miR-based analyses, we compared the expression of miRs (n = 198) between lung cancer cases consuming high versus low quercetin-rich food intake using multivariate ANOVA tests. In family-miR-based analyses, we used Functional Class Scoring (FCS) to assess differential effect on biologically functional miR families. We accounted for multiple testing using 10,000 global permutations (significance at P(global) < 0.10). All multivariate analyses were conducted separately by histology and by smoking status (former and current smokers).
RESULTS: Family-based analyses showed that a quercetin-rich diet differentiated miR expression profiles of the tumor suppressor let-7 family among adenocarcinomas (P(FCS) < 0.001). Other significantly differentiated miR families included carcinogenesis-related miR-146, miR-26, and miR-17 (P (FCS) < 0.05). In individual-based analyses, we found that among former and current smokers with adenocarcinoma, 33 miRs were observed to be differentiated between highest and lowest quercetin-rich food consumers (23 expected by chance; P(global) = 0.047).
CONCLUSIONS: We observed differential expression of key biologically functional miRs between high versus low consumers of quercetin-rich foods in adenocarcinoma cases.
IMPACT: Our findings provide preliminary evidence on the mechanism underlying quercetin-related lung carcinogenesis.
Tram K Lam; Stephanie Shao; Yingdong Zhao; Francesco Marincola; Angela Pesatori; Pier Alberto Bertazzi; Neil E Caporaso; Ena Wang; Maria Teresa Landi
Publication Detail:
Type:  Journal Article     Date:  2012-10-03
Journal Detail:
Title:  Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology     Volume:  21     ISSN:  1538-7755     ISO Abbreviation:  Cancer Epidemiol. Biomarkers Prev.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  2013-08-13     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9200608     Medline TA:  Cancer Epidemiol Biomarkers Prev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2176-84     Citation Subset:  IM    
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MeSH Terms
Adenocarcinoma / epidemiology,  genetics,  metabolism
Carcinoma, Squamous Cell / epidemiology,  genetics,  metabolism
Case-Control Studies
Italy / epidemiology
Lung Neoplasms / epidemiology,  genetics*,  metabolism
MicroRNAs / biosynthesis*,  genetics,  metabolism
Middle Aged
Paraffin Embedding
Quercetin / administration & dosage*
Risk Factors
Tissue Fixation
Grant Support
Reg. No./Substance:
0/MicroRNAs; 1HG84L3525/Formaldehyde; 9IKM0I5T1E/Quercetin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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