Document Detail

Influence of pulsed electromagnetic field with different pulse duty cycles on neurite outgrowth in PC12 rat pheochromocytoma cells.
MedLine Citation:
PMID:  15887254     Owner:  NLM     Status:  MEDLINE    
The influence of low frequency (50 Hz repetition rate) pulsed electromagnetic field (EMF) on PC12 cell neurite outgrowth in vitro was investigated in this study. We studied the percentage of neurite bearing cells, average length of neurites, and directivity of neurite outgrowth in PC12 cells cultured for 96 h in the presence of nerve growth factor (NGF). PC12 cells were exposed in one incubator to pulsed EMF at 1.36 mT (peak value) generated by a pair of Helmholtz coils, and the control samples were placed in another identical incubator. We found that the pulse duty cycle had significant effect on neurite outgrowth. Low (10%) pulse on-time significantly inhibited the percentage of neurite bearing cells, but at the same time increased the average length of neurites, while 100% on-time (DC) had exactly the opposite effects. Furthermore, we found that neurites were prone to extend along the direction of pulsed EMF with 10% pulse on-time. Our studies show that neurite outgrowth in PC12 cells is sensitive to the pulse duty and this sensitivity was associated with NGF concentration.
Y Zhang; J Ding; W Duan; W Fan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioelectromagnetics     Volume:  26     ISSN:  0197-8462     ISO Abbreviation:  Bioelectromagnetics     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-23     Completed Date:  2005-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008281     Medline TA:  Bioelectromagnetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  406-11     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley-Liss, Inc.
Department of Materials Science, National University of Singapore, Singapore.
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MeSH Terms
Cell Enlargement / drug effects*,  radiation effects*
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Electromagnetic Fields*
Nerve Growth Factor / administration & dosage*
Neurites / drug effects*,  pathology,  radiation effects*
PC12 Cells
Radiation Dosage
Reg. No./Substance:
9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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