Document Detail

Influence of the pulpal components on human dentine permeability in vitro.
MedLine Citation:
PMID:  15743418     Owner:  NLM     Status:  MEDLINE    
AIM: To examine the influence of the retained pulpal components on permeability of human dentine by monitoring drug diffusion. METHODOLOGY: Twelve intact dentine discs were prepared from freshly extracted human third molars. The dentine surface on the enamel side was etched with 10% polyacrylic acid for 30 s. The drug diffusion test was carried out before and after removal of the retained pulpal components. Each dentine disc was inserted between two plastic chambers; enamel- and pulpal-side chambers, which were filled with 0.05 mol L(-1) naproxen sodium (NA) and phosphate-buffered saline (PBS), respectively. After 10 min, the solution on the pulpal-side chamber was collected to determine the concentration of NA using a spectrophotometer. To remove the retained pulpal components and residual NA, the pulp chamber of each disc was washed out with PBS and placed in an ultrasonic cleaner. After removal of these components and the residual NA, the drug diffusion test was repeated. The inner surface of the pulp chamber was observed using scanning electron microscopy (SEM) before and after the removal of the retained pulpal components. RESULTS: The amount of NA that diffused through dentine into the pulp was significantly higher after the pulp chamber was washed out with PBS (paired t-test, P < 0.05). SEM observation demonstrated the presence of the retained pulpal components, odontoblastic layer and some parts of subodontoblastic zone, covering the surface of predentine. These components were removed after the pulp chamber was washed out with PBS followed by cleaning in an ultrasonic cleaner. CONCLUSIONS: The presence of retained pulpal components had a significant influence on drug diffusion through dentine discs.
P Puapichartdumrong; H Ikeda; H Suda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International endodontic journal     Volume:  38     ISSN:  0143-2885     ISO Abbreviation:  Int Endod J     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-03     Completed Date:  2005-04-11     Revised Date:  2008-08-07    
Medline Journal Info:
Nlm Unique ID:  8004996     Medline TA:  Int Endod J     Country:  England    
Other Details:
Languages:  eng     Pagination:  152-9     Citation Subset:  D    
Pulp Biology and Endodontics, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / pharmacokinetics*,  therapeutic use
Dental Pulp*
Dentin / metabolism,  ultrastructure
Dentin Permeability*
Microscopy, Electron, Scanning
Naproxen / pharmacokinetics*,  therapeutic use
Pulpitis / drug therapy
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 22204-53-1/Naproxen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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