Document Detail


Influence of prostaglandin I2 on fibronectin-mediated phagocytosis in vivo and in vitro.
MedLine Citation:
PMID:  2981944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study evaluated the effect of prostaglandin I2 (PGI2) on fibronectin-mediated macrophage phagocytosis in vivo and in vitro. Phagocytosis measured in vivo in rats by the vascular clearance rate and hepatic localization gelatinized sheep erythrocytes was inhibited in a dose-dependent manner after intravenous administration of PGI2. Phagocytosis was assessed in vitro in terms of uptake of fibronectin-dependent gelatinized sheep erythrocytes by monolayers of casein-elicited rat peritoneal macrophages. Concentrations of 1 ng/ml PGI2 or greater resulted in inhibition of particle internalization but not attachment to macrophages. This inhibitory effect was enhanced by aminophylline, a phosphodiesterase inhibitor. PGI2 increased cAMP levels and these were further increased in the presence of aminophylline. These data indicate that PGI2 inhibits macrophage uptake of gelatinized particles and support the idea that this is mediated by increased intracellular levels of cyclic AMP. PGI2 should thus be considered a potential etiologic factor in the phagocytic depression observed in association with thrombosis.
Authors:
D A Weinberg; L K Weston; J E Kaplan
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  37     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-02-26     Completed Date:  1985-02-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  151-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aminophylline / pharmacology
Animals
Ascitic Fluid / physiopathology
Cyclic AMP / metabolism
Epoprostenol / administration & dosage,  pharmacology*
Fibronectins / pharmacology*
Injections, Intravenous
Macrophages / drug effects,  metabolism,  physiology*
Male
Mononuclear Phagocyte System / drug effects,  metabolism
Phagocytosis / drug effects*
Rats
Rats, Inbred Strains
Sheep
Grant Support
ID/Acronym/Agency:
GM-00488/GM/NIGMS NIH HHS; GM-25946/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 317-34-0/Aminophylline; 35121-78-9/Epoprostenol; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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