Document Detail

Influence of processing parameters on disintegration of Chlorella cells in various types of homogenizers.
MedLine Citation:
PMID:  18758766     Owner:  NLM     Status:  MEDLINE    
The following bead mills used for disruption of the microalga Chlorella cells were tested: (1) Dyno-Mill ECM-Pilot, grinding chamber volume 1.5 L; KDL-Pilot A, chamber volume 1.4 L; KD 20 S, chamber volume 18.3 L; KD 25 S, chamber volume 26 L of Willy A. Bachofen, Basel, Switzerland, (2) LabStar LS 1, chamber volume 0.6 L of Netzsch, Selb, Germany, (3) MS 18, chamber volume 1.1 L of FrymaKoruma, Neuenburg, Germany. Amount of disrupted cells decreased with increasing Chlorella suspension feed rate and increased up to about 85% of the beads volume in the grinding chamber of the homogenizers. It also increased with agitator speed and number of passes of the algae suspension through the chamber. The optimum beads diameter was 0.3-0.5 mm in the homogenizers Dyno-Mill and LabStar LS 1 and 0.5-0.7 mm in the homogenizer MS 18. While the degree of the cell disruption decreased with increasing cell density in Dyno-Mill and LabStar, the cell disruption in the MS 18 increased. Depending on processing parameters, more than 90% of algae cells were disrupted by passing through the bead mills and bacteria count in algae suspension was reduced to about two orders.
J Doucha; K Lívanský
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-08-29
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  81     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-17     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  431-40     Citation Subset:  IM    
Division of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81, Trebon, Czech Republic.
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MeSH Terms
Cell Fractionation / methods*
Chlorella / chemistry*
Food Handling / methods*
Industrial Microbiology*
Particle Size

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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