Document Detail


Influence of prior freezing on arylamine acetylation by human liver preparations.
MedLine Citation:
PMID:  20692985     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human liver tissue is an important resource for use in xenobiotic metabolism studies. Because of the scarcity of human livers, studies are frequently conducted with subcellular preparations from previously frozen material. The influence of prior freezing on arylamine acetylation with human S-9 was studied and differences were found between S-9 preparations derived from fresh liver and from stored frozen liver. Specifically, the capacity for formation of diacetylated benzidine was lost as a result of tissue freezing, and the rate and extent of monoacetylation appeared to be reduced also, though not as dramatically. These results demonstrate that freezing of human tissue prior to conducting metabolism studies with arylamines may alter metabolite profiles and indicate that, for reliable data, before using frozen human tissue in metabolism studies with arylamines one should check first for this possibility.
Authors:
C E Green; G R Gordon; J H Peters; C A Tyson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  8     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  1994 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  673-5     Citation Subset:  -    
Affiliation:
SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025, USA.
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