| Influence of the presence of OB-Re on leptin radioimmunoassay. | |
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MedLine Citation:
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PMID: 11139772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leptin, a hormone derived from adipose tissue, regulates energy homeostasis and body weight. In the mouse, serum leptin levels, when measured by radioimmunoassay (RIA), increase by a factor of more than 50 times during pregnancy, compared with those in the non-pregnant state. It is well known that mouse placenta produces the secretory isoform of the leptin receptor, OB-Re. In order to investigate the issue of whether serum leptin levels are actually increased during pregnancy or whether the increased OB-Re concentration plays a role in this phenomenon, serum leptin levels were determined by the immunoprecipitation of leptin using anti-leptin antibody, and were found to be increased only by about ten times during pregnancy. To investigate the influence of OB-Re on leptin measurement by the RIA procedure, serum leptin levels were measured by the RIA after the addition of OB-Re to the serum. The apparent values of leptin levels increased in parallel with the amount of OB-Re added to the serum. Leptin levels, as determined by the RIA, might therefore provide artificially high values when serum levels of the secretory form of OB-R are high, in cases, for example, such as the last period of pregnancy in mice. |
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Authors:
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T Murakami; S Otani; T Honjoh; T Doi; K Shima |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of endocrinology Volume: 168 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-02-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 79-86 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, School of Medicine, University of Tokushima, Kuramotocho 3-chome, Tokushima 770-8503, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Blotting, Western Carrier Proteins / blood* Female Half-Life Leptin / blood* Mice Mice, Inbred ICR Precipitin Tests Pregnancy Protein Isoforms Radioimmunoassay Receptors, Cell Surface* Receptors, Leptin Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Leptin; 0/Protein Isoforms; 0/Receptors, Cell Surface; 0/Receptors, Leptin; 0/leptin receptor, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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