Document Detail


Influence of pregnancy on the adipocytokine and PPAR pathways in peripheral blood mononuclear cells of healthy donors and RA patients.
MedLine Citation:
PMID:  22231457     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To identify candidate genes which are regulated by human pregnancy and bear the potential to modulate rheumatoid arthritis (RA) disease activity. METHODS: Peripheral blood mononuclear cells (PBMC) of healthy volunteers (ND) were analyzed using Affymetrix GeneChips at four time points (1(st) , 2(nd) and 3(rd) trimester and 6 weeks postpartum). Based on the GeneChip data, target genes were further analyzed via quantitative real-time PCR (qPCR) using PBMC from ND and RA patients. In order to determine the cellular source, monocytes and lymphocytes from ND and RA patients were positively selected using magnetic beads and their mRNA was analyzed by qPCR. RESULTS: One-way ANOVA analysis identified 1286 mRNAs differentially expressed with regard to the four time points. The changes became more pronounced as pregnancy progressed and they were reverted postpartum. A subsequent pathway analysis suggested a regulatory role of pregnancy on the adipocytokine pathway as well as the PPAR-signaling pathway. Of 19 pre-selected candidate genes, AKT3, SOCS3, FADS2, STAT1 and CD36 proofed to be differentially regulated by pregnancy. In samples of RA patients the differences were concordant but more pronounced. Both, T lymphocytes and monocytes contributed to the regulated expression of these genes. CONCLUSIONS: Normal human pregnancy leads to changes in the expression level of several molecular pathways in PBMC which are reverted postpartum. Changes in RA, though concordant, exceed levels observed in ND. Genes of the adipocytokine- and the PPAR-signaling pathways qualify as candidates for the modulation of RA disease activity during pregnancy.
Authors:
Janine Weix; Frauke Förger; Thomas Häupl; Daniel Surbek; Monika Ostensen; Peter M Villiger
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-9
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  -     ISSN:  1529-0131     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 by the American College of Rheumatology.
Affiliation:
Department of Rheumatology, Clinical Immunology and Allergology, University Hospital and University of Bern, Switzerland.
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