Document Detail

Influence of prednisolone on the secretion of mucin from the HT29-MTX cell line.
MedLine Citation:
PMID:  12580879     Owner:  NLM     Status:  MEDLINE    
Glucocorticoids have been used in the treatment of otitis media with effusion with promising but inconsistent results. The HT29-MTX cell line is a completely differentiated and almost exclusively mucus-secreting goblet cell line. To assess the potential of steroids in suppressing mucin secretion, we have studied the response of this cell culture to prednisolone. Confluent cell cultures were trypsinized, subcultured in six-well plates and incubated with five doses of prednisolone from 10-3 M to 10-11 M and over a varying time course from 6 to 36 h. Analysis was performed using a monoclonal mouse antibody to human gastric mucin by dot-blot ELISA. Prednisolone caused a consistent reduction in mucin production from this cell line. Increasing concentrations of prednisolone resulted in increasing suppression of MUC5AC secretion. There is a dose-dependent suppression of mucin secretion by prednisolone, with a maximum effect of 21% over control seen at the highest steroid concentration used.
N Fergie; L Guo; J Sithole; J P Pearson; J P Birchall
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical otolaryngology and allied sciences     Volume:  28     ISSN:  0307-7772     ISO Abbreviation:  Clin Otolaryngol Allied Sci     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-12     Completed Date:  2003-05-28     Revised Date:  2005-05-02    
Medline Journal Info:
Nlm Unique ID:  7701793     Medline TA:  Clin Otolaryngol Allied Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  39-42     Citation Subset:  IM    
Department of Otolaryngology and Head and Neck Surgery, Queen's Medical Centre, Nottingham, UK.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology*
Antibodies, Monoclonal / metabolism
Cell Culture Techniques
Cell Differentiation
Enzyme-Linked Immunosorbent Assay
Gastric Mucosa / metabolism,  pathology
Goblet Cells / metabolism,  pathology
HT29 Cells / drug effects*,  metabolism*,  pathology
Mucins / metabolism*
Otitis Media with Effusion / metabolism,  pathology
Prednisolone / pharmacology*
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Antibodies, Monoclonal; 0/Mucins; 50-24-8/Prednisolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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