| Influence of prednisolone on the secretion of mucin from the HT29-MTX cell line. | |
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MedLine Citation:
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PMID: 12580879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucocorticoids have been used in the treatment of otitis media with effusion with promising but inconsistent results. The HT29-MTX cell line is a completely differentiated and almost exclusively mucus-secreting goblet cell line. To assess the potential of steroids in suppressing mucin secretion, we have studied the response of this cell culture to prednisolone. Confluent cell cultures were trypsinized, subcultured in six-well plates and incubated with five doses of prednisolone from 10-3 M to 10-11 M and over a varying time course from 6 to 36 h. Analysis was performed using a monoclonal mouse antibody to human gastric mucin by dot-blot ELISA. Prednisolone caused a consistent reduction in mucin production from this cell line. Increasing concentrations of prednisolone resulted in increasing suppression of MUC5AC secretion. There is a dose-dependent suppression of mucin secretion by prednisolone, with a maximum effect of 21% over control seen at the highest steroid concentration used. |
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Authors:
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N Fergie; L Guo; J Sithole; J P Pearson; J P Birchall |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical otolaryngology and allied sciences Volume: 28 ISSN: 0307-7772 ISO Abbreviation: Clin Otolaryngol Allied Sci Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-12 Completed Date: 2003-05-28 Revised Date: 2005-05-02 |
Medline Journal Info:
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Nlm Unique ID: 7701793 Medline TA: Clin Otolaryngol Allied Sci Country: England |
Other Details:
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Languages: eng Pagination: 39-42 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology and Head and Neck Surgery, Queen's Medical Centre, Nottingham, UK. neil.fergie@virgin.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents
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pharmacology* Antibodies, Monoclonal / metabolism Cell Culture Techniques Cell Differentiation Enzyme-Linked Immunosorbent Assay Gastric Mucosa / metabolism, pathology Goblet Cells / metabolism, pathology HT29 Cells / drug effects*, metabolism*, pathology Humans Mucins / metabolism* Otitis Media with Effusion / metabolism, pathology Prednisolone / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Antibodies, Monoclonal; 0/Mucins; 50-24-8/Prednisolone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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