Document Detail


Influence of precursors and inhibitor on the production of extracellular 5-aminolevulinic acid and biomass by Rhodopseudomonas palustris KG31.
MedLine Citation:
PMID:  19420716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
5-Aminolevulinic acid (ALA) and the biomass of photosynthetic bacteria, Rhodopseudomonas palustris KG31, have very high potential for development and exploitation as bioherbicide and biofertilizer respectively. In this work, the effects of two precursors and an inhibitor of aminolevulinic dehydratase (ALAD) added to the VFA culture medium on the production of ALA and biomass were investigated. The experimental runs were carried out according to a Box-Behnken design. The precursors were added to the medium at the beginning of cultivation, while the inhibitor was added after 24 h. Statistical analysis indicated that levulinic acid (LA) has a positive effect on ALA production while glycine has a negative effect on biomass production. In order to enhance both ALA and biomass products, the most suitable medium was VFA medium supplemented with 3.0 mM glycine and 10 mM LA, giving ALA and biomass of 182.91 microM and 3.1 gDCW/l within 54 h.
Authors:
Angkana Saikeur; Wanna Choorit; Poonsuk Prasertsan; Duangporn Kantachote; Ken Sasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  987-92     Citation Subset:  IM    
Affiliation:
School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Aminolevulinic Acid / metabolism*
Biomass*
Enzyme Inhibitors / pharmacology
Extracellular Space / drug effects*,  metabolism*
Glycine / pharmacology
Levulinic Acids / pharmacology
Models, Biological
Porphobilinogen Synthase / antagonists & inhibitors
Rhodopseudomonas / drug effects*,  metabolism*
Succinic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Levulinic Acids; 106-60-5/Aminolevulinic Acid; 110-15-6/Succinic Acid; 123-76-2/levulinic acid; 56-40-6/Glycine; EC 4.2.1.24/Porphobilinogen Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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