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Influence of poly(n-isopropylacrylamide)-CNT-polyaniline three-dimensional electrospun microfabric scaffolds on cell growth and viability.
MedLine Citation:
PMID:  23426576     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)-CNT-polyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)-CNT-polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400-500 μm; L: 6-20 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)-CNT-polyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)-CNT-polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)-CNT-polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)-CNT-polyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 334-341, 2013.
Authors:
Ashutosh Tiwari; Yashpal Sharma; Shinya Hattori; Dohiko Terada; Ashok K Sharma; Anthony P F Turner; Hisatoshi Kobayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biopolymers     Volume:  99     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-02-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  334-41     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, S-58183 Linköping, Sweden; International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305-0047, Japan. ashutosh.tiwari@liu.se.
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