Document Detail

Influence of the physical states of membrane surface area and center area on lysosomal proton permeability.
MedLine Citation:
PMID:  12147267     Owner:  NLM     Status:  MEDLINE    
The physical state of the lysosomal membrane was modulated with the membrane fluidizers n-propanol and n-octanol and with the membrane rigidifiers cholesteryl hemisuccinate and cholesterol. Membrane fluidity was examined by the steady-state fluorescence anisotropy of 2-(9-anthroyloxy) palmitic acid and 16-(9-anthroyloxy) palmitic acid. Fluidizing the membranes at the surface and center areas increased the proton permeability coefficient by 92.8 and 18.0%, respectively. Rigidifying the membranes at the surface and center areas decreased the coefficient by 68.2 and 40.2%, respectively. Proton leakage of the lysosomes increased and decreased similar to the coefficient changes with the treatments. The results indicate that lysosomal proton permeability is affected by its membrane's physical state, and the physical state of the membrane surface area affects the proton permeability more markedly. The proton permeability coefficient of liposomes was similar to that of lysosomes, suggesting that efflux of lysosomal protons might occur through the lipid part of the bilayer but not transmembrane proteins.
Feng-Yi Wan; Yi-Nan Wang; Guo-Jiang Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  404     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2002-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-92     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science (USA).
Center for Molecular Biology, Institute of Biophysics, Academia Sinica, Beijing 100101, People's Republic of China.
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MeSH Terms
1-Octanol / pharmacology
1-Propanol / pharmacology
Cholesterol / pharmacology
Cholesterol Esters / pharmacology
Fluorescence Polarization
Fluorescent Dyes
Intracellular Membranes / chemistry*,  drug effects
Liver / chemistry
Lysosomes / chemistry*
Membrane Fluidity / drug effects,  physiology
Palmitic Acids / chemistry
Rats, Wistar
Surface Properties
Reg. No./Substance:
0/Cholesterol Esters; 0/Fluorescent Dyes; 0/Palmitic Acids; 0/Protons; 111-87-5/1-Octanol; 1510-21-0/cholesteryl succinate; 57-88-5/Cholesterol; 64821-29-0/16-(9-anthroyloxy)palmitic acid; 67708-96-7/2-(9-anthroyloxy)palmitate; 71-23-8/1-Propanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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