| Influence of phospholipid composition on the properties of reconstituted surfactants. | |
| | |
MedLine Citation:
|
PMID: 10192760 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The influence of phospholipids on the ultrastructure and metabolism of reconstituted surfactants has not been well defined. The aim of this study was to determine if changes in the phospholipid composition of reconstituted surfactants altered their biophysical properties, ultrastructure, and conversion to light subtype by cycling. We prepared various surfactants containing radiolabeled dipalmitoylphosphatidylcholine ([14C]DPPC). The addition of phosphatidylglycerol (PG) or dipalmitoylphosphatidic acid (PA) to DPPC increased conversion to light subtype. In contrast, the addition of dipalmitoylphosphatidylglycerol (DPPG) to DPPC markedly reduced conversion to light subtype on cycling. DPPC and DPPC+PG produced large liposomes ( approximately 1,000 nm), whereas DPPC+PA or DPPC+DPPG formed multilamellar membranes. Mixtures of DPPC and PA were highly surface active in vitro, whereas the surface activity of DPPC+DPPG was similar to that of DPPC. In conclusion, the ultrastructure, metabolism, and surface active properties of DPPC+PG mixtures were influenced markedly by alterations in the fatty acid composition or polar head group of PG. |
| | |
Authors:
|
R Dhand; J Young; S Krishnasamy; F Possmayer; N J Gross |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Lung Volume: 177 ISSN: 0341-2040 ISO Abbreviation: Lung Publication Date: 1999 |
Date Detail:
|
Created Date: 1999-05-17 Completed Date: 1999-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7701875 Medline TA: Lung Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 127-38 Citation Subset: IM |
Affiliation:
|
Division of Pulmonary and Critical Care Medicine, Edward Hines Jr. Veterans Affairs Hospital and Loyola University of Chicago Stritch School of Medicine, Hines, IL 60141, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1,2-Dipalmitoylphosphatidylcholine
/
analysis*,
pharmacology Carbon Radioisotopes Humans Microscopy, Electron Phosphatidylglycerols Phospholipids / analysis* Pulmonary Surfactants / chemistry, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Radioisotopes; 0/Phosphatidylglycerols; 0/Phospholipids; 0/Pulmonary Surfactants; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; 4537-77-3/1,2-dipalmitoylphosphatidylglycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of laxatives in institutions for the mentally retarded.
Next Document: 1,25-dihydroxycholecalciferol stimulates ICAM-1 expression of human alveolar macrophages in healthy ...