Document Detail


Influence of phenylalanines 77 and 138 on the stereospecificity of aryl sulfotransferase IV.
MedLine Citation:
PMID:  15100179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aryl sulfotransferase (AST) IV (also named tyrosine-ester sulfotransferase and ST1A1) is a major phenol sulfotransferase in the rat, and it catalyzes the sulfation of many drugs, carcinogens, and other xenobiotics that contain phenol, benzylic alcohol, N-hydroxy arylamine, and oxime functional groups. Previous work discovered a stereospecificity of AST IV toward the enantiomers of 1,2,3,4-tetrahydro-1-naphthol and varying degrees of stereoselectivity with other chiral benzylic alcohols. The studies described here were directed toward understanding the roles of specific amino acid residues at the substrate binding site in determining the stereoselectivity of this sulfotransferase isoform. Docking experiments with a homology model of AST IV revealed three amino acid residues, Phe77, Phe138, and Tyr236, that may potentially be important for interactions with substituents on the chiral carbon of a benzylic alcohol serving as a sulfuryl acceptor, thereby imparting stereoselectivity. To test this hypothesis, mutants were constructed wherein each of the above residues was substituted with alanine. Kinetic studies on the sulfation of the enantiomers of 1,2,3,4-tetrahydro-1-naphthol indicated that the stereospecificity of the sulfotransferase was altered by the substitutions of alanine for either Phe77 or Phe138, but stereospecificity was maintained by alanine substitution at Tyr236. Molecular models of the mutant enzymes interacting with enantiomers of 1,2,3,4-tetrahydro-1-naphthols and with 2-naphthol indicate that Phe77 and Phe138 provide significant steric interactions at the active site that both enhance catalytic efficiency and impart stereospecificity in molecular recognition of substrates and inhibitors.
Authors:
Jonathan J Sheng; Atmaja Saxena; Michael W Duffel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Drug metabolism and disposition: the biological fate of chemicals     Volume:  32     ISSN:  0090-9556     ISO Abbreviation:  Drug Metab. Dispos.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-21     Completed Date:  2004-12-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421550     Medline TA:  Drug Metab Dispos     Country:  United States    
Other Details:
Languages:  eng     Pagination:  559-65     Citation Subset:  IM    
Affiliation:
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Binding Sites
Catalysis
Molecular Sequence Data
Phenylalanine / chemistry*,  genetics
Rats
Stereoisomerism
Sulfotransferases / chemistry*,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
CA 38683/CA/NCI NIH HHS; F32 CA 91704/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
63-91-2/Phenylalanine; EC 2.8.2.-/Sulfotransferases; EC 2.8.2.9/tyrosine-ester sulfotransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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