Document Detail

Influence of peptide supply and cosubstrates on phenylalanine metabolism of Lactobacillus sanfranciscensis DSM20451(T) and Lactobacillus plantarum TMW1.468.
MedLine Citation:
PMID:  16719504     Owner:  NLM     Status:  MEDLINE    
Bread spoilage is mainly due to the growth of filamentous fungi, and metabolites produced during sourdough fermentation by lactobacilli can inhibit fungal growth. One of these metabolites is phenyllactic acid (PLA), which is a catabolite from phenylalanine. In this work, the influence of peptide supply and cosubstrates was determined on PLA formation from phenylalanine by Lactobacillus plantarum TMW1.468 and Lactobacillus sanfranciscensis DSM20451(T). Transport of single amino acids is not efficient in lactobacilli, and only 1% of the offered phenylalanine was converted to PLA. PLA yields were increased 2-4-fold when peptides instead of single amino acids were used as a substrate. The accumulation of phenylalanine after peptide addition indicated that, after transport, transamination was the second limiting factor. In L. plantarum TMW1.468, PLA yields were increased from 5 to >30% upon the addition of alpha-ketoglutarate. In L. sanfranciscensis DSM20451, a combination of both citric acid and alpha-ketoglutarate increased PLA formation. The combined effect of citric acid and alpha-ketoglutarate can be attributed to changes in the NAD/NADH ratio.
Nicoline Vermeulen; Michael G Ganzle; Rudi F Vogel
Related Documents :
17538924 - Amino acids: aspects of impurity profiling by means of ce.
7629064 - Proofreading in vivo. editing of homocysteine by aminoacyl-trna synthetases in escheric...
1297114 - New proctolin analogs modified in position 3 of peptide chain--synthesis and their biol...
1454354 - The evolution of aminoacyl-trna synthetases, the biosynthetic pathways of amino acids a...
21598174 - Physico-chemical comparison of betulinic acid, betulin and birch bark extract and in v...
15406374 - Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the r...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-24     Completed Date:  2006-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3832-9     Citation Subset:  IM    
Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, D-85350 Freising, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids, Branched-Chain / pharmacology
Bread / analysis
Ketoglutaric Acids / pharmacology
Lactobacillus / metabolism*
Lactobacillus plantarum / metabolism*
Peptides / analysis,  metabolism*
Phenylacetates / metabolism*
Phenylalanine / analysis,  metabolism*
Reg. No./Substance:
0/Amino Acids, Branched-Chain; 0/Ketoglutaric Acids; 0/Peptides; 0/Phenylacetates; 103-82-2/phenylacetic acid; 328-50-7/alpha-ketoglutaric acid; 63-91-2/Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evaluation of the antioxidant capacity of limonin, nomilin, and limonin glucoside.
Next Document:  Lactobacillus plantarum and Pediococcus pentosaceus starter cultures as a tool for microflora manage...