Document Detail


Influence of pentoxifylline on membrane thrombomodulin levels in endothelial cells submitted to hypoxic conditions.
MedLine Citation:
PMID:  8699869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thrombomodulin (TM) expression at the surface of endothelial cells is upregulated by cAMP analogues, whereas hypoxic conditions induce a decrease in this expression. Pentoxifylline (PTX) enhances the intracellular levels of cAMP in human umbilical vein endothelial cells (HUVECs). We therefore investigated the influence of this drug on TM expression in cells submitted to hypoxia. TM expression was quantified at the surface of HUVECs in the presence or absence of PTX during 18 h of incubation. Membrane TM was immunolabeled with a fluorescent monoclonal antibody and the expression was quantified by flow cytometric analysis. Our preliminary data revealed that PTX at 0.01 microM significantly increased TM expression levels compared with cells in hypoxia without the drug. Therefore, at this low dose PTX appears able to counterbalance the procoagulant effect of hypoxia.
Authors:
M Seigneur; P Dufourcq; F Belloc; M Lenoble; M Renard; M R Boisseau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  25 Suppl 2     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  1995  
Date Detail:
Created Date:  1996-09-05     Completed Date:  1996-09-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S85-7     Citation Subset:  IM    
Affiliation:
Laboratory of Hematology, University of Bordeaux II, Bordeaux, France.
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MeSH Terms
Descriptor/Qualifier:
Anoxia / metabolism*
Cell Membrane / drug effects,  metabolism
Cells, Cultured
Endothelium, Vascular / cytology,  drug effects,  metabolism*
Flow Cytometry
Humans
Pentoxifylline / pharmacology*
Phosphodiesterase Inhibitors / pharmacology*
Thrombomodulin / metabolism*
Chemical
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 0/Thrombomodulin; 6493-05-6/Pentoxifylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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