Document Detail


Influence of parasite load on the ability of type 1 T cells to control Leishmania major infection.
MedLine Citation:
PMID:  11796575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BALB/c mice infected with Leishmania major developed a type 2 immune response which failed to control parasite replication. We found that scid mice that received splenocytes from BALB/c mice that had been infected for 3 weeks with L. major (a type 2 cell population) and that were subsequently infected with L. major were protected when they were treated with interleukin 12 (IL-12). In contrast, IL-12 was ineffective at protecting BALB/c mice infected for 3 weeks, suggesting that a high parasite load regulates the development of protective immunity. To determine how this regulation operates, we performed a series of adoptive transfers of naïve, type 1 or type 2 splenocytes into scid mice. The recipient scid mice were infected either for 5 weeks prior to cell transfer (and thus had a high parasite load) or at the time of cell transfer. scid mice that were infected for 5 weeks and received a type 1 cell population were able to cure their lesions. However, when 5-week-infected scid mice received both type 1 and 2 cell populations, they were unable to control their infections. In contrast, the same type 1 and 2 cells transferred to naïve scid mice, which were subsequently infected, provided protection. In addition, we found that naïve cells mediated protection in scid mice with established lesions. These results show that high parasite numbers do not block type 1 protective responses or the development of type 1 responses. Instead, the influence of a high parasite load is dependent on the presence of a type 2 cell population.
Authors:
Brian Hondowicz; Phillip Scott
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  70     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-17     Completed Date:  2002-02-21     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  498-503     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chronic Disease
Female
Interleukin-12 / administration & dosage,  immunology
Leishmania major / growth & development*,  immunology
Leishmaniasis, Cutaneous / blood,  immunology,  parasitology*,  pathology
Lymphocyte Count
Mice
Mice, Inbred BALB C
Mice, SCID
Th1 Cells / cytology,  immunology*
Th2 Cells / cytology
Time Factors
Grant Support
ID/Acronym/Agency:
AI 35914/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
187348-17-0/Interleukin-12
Comments/Corrections

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