Document Detail


Influence of p53 expression on sensitivity of cancer cells to bleomycin.
MedLine Citation:
PMID:  20135637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we determined whether p53 expression affected the sensitivity of non-small cell lung cancer (NSCLC) and colon cancer cells to bleomycin (BLM). Two human NSCLC cell lines (A549 expressing wild-type p53 and p53-null H1299) and two colon cancer cell lines (HCT116 p53+/+ and its p53 deficient subline HCT116 p53-/-) were subjected to treatment with BLM. Cells were treated with various concentrations of BLM, and cellular viability was assessed by formazan assay. To investigate the role of p53 in BLM sensitivity, we transduced cells with adenovirus with wild-type p53, dominant-negative p53, and GFP control, and analyzed the effect on cellular viability. Cells expressing wild-type p53 were more sensitive to BLM than p53-null cells in both NSCLC and colon cancer cells. Sensitivity to BLM of the cells with wild-type p53 was reduced by overexpression of dominant-negative p53, while BLM sensitivity of p53-null cells was increased by wild-type p53 in both NSCLC cells and colon cancer cells. In conclusion, our data show that p53 sensitizes all four cancer cells lines tested to BLM toxicity and overexpression of p53 confers sensitivity to the cytotoxic activity of the anticancer agent in p53-null cells.
Authors:
Young Sook Lee; Seokjoo Yoon; Mi Sun Park; Jin Hwa Kim; Jeung-Hoon Lee; Chang-Woo Song
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemical and molecular toxicology     Volume:  24     ISSN:  1099-0461     ISO Abbreviation:  J. Biochem. Mol. Toxicol.     Publication Date:    2010 Jul-Aug
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-12-16     Revised Date:  2011-03-18    
Medline Journal Info:
Nlm Unique ID:  9717231     Medline TA:  J Biochem Mol Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  260-9     Citation Subset:  IM    
Affiliation:
Toxicogenomics Team, Korea Institute of Toxicology, Daejeon 305-343, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antibiotics, Antineoplastic / pharmacology*
Bleomycin / pharmacology*
Carcinoma, Non-Small-Cell Lung / genetics,  metabolism*
Cell Line, Tumor
Cell Survival / drug effects,  genetics
Colonic Neoplasms / genetics,  metabolism*
Drug Resistance, Neoplasm / drug effects,  genetics
Gene Expression Regulation, Neoplastic / drug effects*
Humans
Lung Neoplasms / genetics,  metabolism*
Tumor Suppressor Protein p53 / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53; 11056-06-7/Bleomycin

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