Document Detail


Influence of norcantharidin on proliferation, proliferation-related gene proteins proliferating cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells.
MedLine Citation:
PMID:  15567755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be considered for palliative treatment such as chemotherapy and radiotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carcinoma were cultured by the cell culture technique. The experiment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose-and time-dependent manner, with the IC50 value of 56.18 microg/ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932+/-0.031 vs. 0.318+/-0.023, P<0.001) and Ki-67 (0.964+/-0.092 vs. 0.297+/-0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expression of their proliferation-related gene proteins PCNA and Ki-67.
Authors:
Yue-Zu Fan; Jin-Ye Fu; Ze-Ming Zhao; Cun-Qiu Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hepatobiliary & pancreatic diseases international : HBPD INT     Volume:  3     ISSN:  1499-3872     ISO Abbreviation:  HBPD INT     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-29     Completed Date:  2005-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101151457     Medline TA:  Hepatobiliary Pancreat Dis Int     Country:  China    
Other Details:
Languages:  eng     Pagination:  603-7     Citation Subset:  IM    
Affiliation:
Department of Surgery, Tongji Hospital of Tongji University, 389 Xincun Road, Shanghai 200065, China. fanyuezu_shtj@msn.com
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MeSH Terms
Descriptor/Qualifier:
Bicyclo Compounds, Heterocyclic / pharmacology*
Carcinoma / metabolism*,  pathology*
Cell Line, Tumor
Cell Proliferation / drug effects
Gallbladder Neoplasms / metabolism*,  pathology*
Humans
Immunohistochemistry / methods
Ki-67 Antigen / metabolism*
Proliferating Cell Nuclear Antigen / metabolism*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Bicyclo Compounds, Heterocyclic; 0/Ki-67 Antigen; 0/Proliferating Cell Nuclear Antigen; 5442-12-6/norcantharidin

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