Document Detail

Influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase.
MedLine Citation:
PMID:  2546826     Owner:  NLM     Status:  MEDLINE    
Bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. Palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microM, when measured using a spectrophotometric procedure but not with a polarographic assay method. At higher fatty acid concentrations no further change was found. A 50% decrease in respiratory control was determined when the enzyme was reconstituted in pure phosphatidylcholine containing 2% cardiolipin. The respiratory control of reconstituted cytochrome-c oxidase from bovine liver was not influenced by fatty acids.
C Thiel; B Kadenbach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  251     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-08-29     Completed Date:  1989-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  270-4     Citation Subset:  IM    
Fachbereich Chemie, Philipps-Universität, Marburg, FRG.
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MeSH Terms
Cytochrome c Group / metabolism
Electron Transport Complex IV / metabolism*
Fatty Acids, Nonesterified / pharmacology*
Liposomes / metabolism
Mitochondria, Heart / enzymology
Mitochondria, Liver / enzymology
Oxygen Consumption*
Palmitic Acid
Palmitic Acids / pharmacology
Stearic Acids / pharmacology
Reg. No./Substance:
0/Cardiolipins; 0/Cytochrome c Group; 0/Fatty Acids, Nonesterified; 0/Liposomes; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Stearic Acids; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; EC Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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