| Influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase. | |
| | |
MedLine Citation:
|
PMID: 2546826 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. Palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microM, when measured using a spectrophotometric procedure but not with a polarographic assay method. At higher fatty acid concentrations no further change was found. A 50% decrease in respiratory control was determined when the enzyme was reconstituted in pure phosphatidylcholine containing 2% cardiolipin. The respiratory control of reconstituted cytochrome-c oxidase from bovine liver was not influenced by fatty acids. |
| | |
Authors:
|
C Thiel; B Kadenbach |
Related Documents
:
|
9304806 - Characterization and cytotoxicity of l-amino acid oxidase from the venom of king cobra ... 17542496 - L-amino acid oxidases with specificity for basic l-amino acids in cyanobacteria. 14580796 - Catabolism of volatile sulfur compounds precursors by brevibacterium linens and geotric... 2366876 - Modulation by drugs of the release of total tritium and 3h-5-ht from rat hypothalamic s... 1259536 - Molecular size and amino acid composition of h-2d antigen solubilized in nonidet p-40. 6133566 - Effects of excess amounts of synthetic amino acid preparations on hypothalamus of mice ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: FEBS letters Volume: 251 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1989 Jul |
Date Detail:
|
Created Date: 1989-08-29 Completed Date: 1989-08-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 270-4 Citation Subset: IM |
Affiliation:
|
Fachbereich Chemie, Philipps-Universität, Marburg, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cardiolipins Cattle Cytochrome c Group / metabolism Electron Transport Complex IV / metabolism* Fatty Acids, Nonesterified / pharmacology* Liposomes / metabolism Mitochondria, Heart / enzymology Mitochondria, Liver / enzymology Oxidation-Reduction Oxygen Consumption* Palmitic Acid Palmitic Acids / pharmacology Phosphatidylcholines Spectrophotometry Stearic Acids / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Cardiolipins; 0/Cytochrome c Group; 0/Fatty Acids, Nonesterified; 0/Liposomes; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Stearic Acids; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of hepatic expression of IGF I and fetal IGF binding protein mRNA in streptozotocin-diabe...
Next Document: Na+/H+ exchange in mitochondria as monitored by BCECF fluorescence.