Document Detail

Influence of nitrate on oxalate- and glyoxylate-dependent growth and acetogenesis by Moorella thermoacetica.
MedLine Citation:
PMID:  12420166     Owner:  NLM     Status:  MEDLINE    
Oxalate and glyoxylate supported growth and acetate synthesis by Moorella thermoacetica in the presence of nitrate under basal (without yeast extract) culture conditions. In oxalate cultures, acetate formation occurred concomitant with growth and nitrate was reduced in the stationary phase. Growth in the presence of [(14)C]bicarbonate or [(14)C]oxalate showed that CO(2) reduction to acetate and biomass or oxalate oxidation to CO(2) was not affected by nitrate. However, cells engaged in oxalate-dependent acetogenesis in the presence of nitrate lacked a membranous b-type cytochrome, which was present in cells grown in the absence of nitrate. In glyoxylate cultures, growth was coupled to nitrate reduction and acetate was formed in the stationary phase after nitrate was totally consumed. In the absence of nitrate, glyoxylate-grown cells incorporated less CO(2) into biomass than oxalate-grown cells. CO(2) conversion to biomass by glyoxylate-grown cells decreased when cells were grown in the presence of nitrate. These results suggest that: (1) oxalate-grown cells prefer CO(2) as an electron sink and bypass the nitrate block on the acetyl-CoA pathway at the level of reductant flow and (2) glyoxylate-grown cells prefer nitrate as an electron sink and bypass the nitrate block of the acetyl-CoA pathway by assimilating carbon via an unknown process that supplements or replaces the acetyl-CoA pathway. In this regard, enzymes of known pathways for the assimilation of two-carbon compounds were not detected in glyoxylate- or oxalate-grown cells.
Corinna Seifritz; Jürgen M Fröstl; Harold L Drake; Steven L Daniel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-09-04
Journal Detail:
Title:  Archives of microbiology     Volume:  178     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-06     Completed Date:  2003-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  457-64     Citation Subset:  IM    
Lehrstuhl für Okologische Mikrobiologie, BITOK, Universität Bayreuth, 95440 Bayreuth, Germany.
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MeSH Terms
Carbon Dioxide / metabolism*
Electron Transport / drug effects
Glyoxylates / metabolism
Gram-Positive Bacteria / drug effects*,  growth & development,  metabolism
Nitrates / pharmacology*
Oxalates / metabolism
Reg. No./Substance:
0/Glyoxylates; 0/Nitrates; 0/Oxalates; 124-38-9/Carbon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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