Document Detail


Influence of nematode age and culture conditions on morphological and physiological parameters in the bacterial vesicle of Steinernema carpocapsae (Nematoda: Steinernematidae).
MedLine Citation:
PMID:  17376477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Steinernema spp. third-stage infective juveniles (IJs) play a key role in the symbiotic partnership between these entomopathogenic nematodes and Xenorhabdus bacteria. Recent studies suggest that Steinernema carpocapsae IJs contribute to the nutrition and growth of their symbionts in the colonization site (vesicle) [Martens, E.C. and Goodrich-Blair, H., 2005. The S. carpocapsae intestinal vesicle contains a sub-cellular structure with which Xenorhabdus nematophila associates during colonization initiation. Cellular Microbiol. 7, 1723-1735.]. However, the morphological and physiological interactions between Xenorhabdus symbionts and Steinernema IJs are not understood in depth. This study was undertaken to assess the influence of culture conditions and IJ age on the structure, nutrition, and symbiont load (colonization level) of S. carpocapsae vesicles. Our observations indicate the vesicles of axenic IJs are shorter and wider than those of colonized IJs. Moreover, as colonized IJs age the vesicle becomes shorter and narrower and bacterial load declines. The colonization proficiency of several bacterial metabolic mutants was compared between two cultivation conditions: in vitro on lipid agar and in vivo in Galleria mellonella insects. Colonization defects were generally less severe in IJs cultivated in vivo versus those cultivated in vitro. However, IJs from both cultivation conditions exhibited similar declining bacterial load over time. These results suggest that although the vesicle forms in the absence of bacteria, the presence of symbionts within the vesicle may influence its fine structure. Moreover, these studies provide further evidence in support of the concept that the conditions under which steinernematid nematodes are cultivated and stored affect the nutritive content of the vesicle and the bacterial load, and therefore have an impact on the quality of the nematodes for their application as biological control agents.
Authors:
Yolanda Flores-Lara; Darby Renneckar; Steven Forst; Heidi Goodrich-Blair; Patricia Stock
Related Documents :
9788457 - Interleukin-1 receptor antagonist and interleukin-6 for early diagnosis of neonatal sep...
21866057 - Practical handling, ease of use, safety, and efficacy of a new pediatric triple-chamber...
21297707 - Acquired bacterial infection in the newborn.
10838717 - Clinico-bacteriological study of neonatal septicemia in hubli.
9788457 - Interleukin-1 receptor antagonist and interleukin-6 for early diagnosis of neonatal sep...
7294007 - Periodic breathing in infants with histories of prolonged apnea.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-02-03
Journal Detail:
Title:  Journal of invertebrate pathology     Volume:  95     ISSN:  0022-2011     ISO Abbreviation:  J. Invertebr. Pathol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-09-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0014067     Medline TA:  J Invertebr Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  110-8     Citation Subset:  IM    
Affiliation:
Department of Entomology, University of Arizona, Forbes Bldg, Tucson, AZ 85721-0036, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Cell Culture Techniques
Host-Parasite Interactions / physiology*
Nematode Infections / microbiology*
Pest Control, Biological
Rhabditida / microbiology*,  ultrastructure
Symbiosis / physiology*
Xenorhabdus / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ascorbic acid as a mediator and template for assembling metallic nanoparticles.
Next Document:  The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Try...