Document Detail


Influence of nanometer smoothness and fibronectin immobilization of titanium surface on MC3T3-E1 cell behavior.
MedLine Citation:
PMID:  22447768     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of the present study was to evaluate the influence of mechanical treatment, namely, nanometer smoothing (Ra: approximately 2.0 nm) and sandblasting (Ra: approximately 1.0 μm), as well as biochemical treatment, namely, fibronectin immobilization, of a titanium surface on osteoblast-like cell behavior. Cell proliferation was monitored by measurements of DNA content and ALP activity; osteocalcin production and mineralization behavior were also evaluated, in addition to morphological observation of attached cells. Fibronectin could be immobilized by the tresyl chloride-activation method. A sandblasted surface resulted in significantly more DNA than a nanometer-smooth surface, but fibronectin immobilization did not result in a significant increase of DNA at 52 days of cell culture. The nanometer-smooth surface showed highest ALP activity and osteocalcin production. FN immobilization decreased ALP activity for the nanometer-smooth surface, but increased it for the sandblasted surface. The nanometer-smooth surface also showed the highest osteocalcin production. Scanning electron microscopy showed interesting phenomena of the attached cells. Attached cell area was more rapidly increased on the nanometer-smooth surface than on the sandblasted surface. It was suggested that cultured cells on the nanometer-smooth surface began to spread earlier and that the proportion of spreading cells among total attached cells increased sooner on the nanometer-smooth surface than on the sandblasted rough surface. It appeared that FN immobilization influenced the arrangement of attached cells. In conclusion, the nanometer-smooth surface employed in the present study was beneficial for the differentiation of MC3T3-E1 cells. FN immobilization influenced the morphologies of attached cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.
Authors:
Eiji Yoshida; Yoshitaka Yoshimura; Motohiro Uo; Masao Yoshinari; Tohru Hayakawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-23
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Dental Engineering, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-Ku, Yokohama 230-8501, Japan. yoshida-e@tsurumi-u.ac.jp.
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