Document Detail


Influence of microvascular endothelial cells on transcriptional regulation of proximal tubular epithelial cells.
MedLine Citation:
PMID:  18057119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the renal cortex the peritubular capillary network and the proximal tubular epithelium cooperate in solute and water reabsorption, secretion, and inflammation. However, the mechanisms by which these two cell types coordinate such diverse functions remain to be characterized. Here we investigated the influence of microvascular endothelial cells on proximal tubule cells, using a filter-based, noncontact, close-proximity coculture of the human microvascular endothelial cell line HMEC-1 and the human proximal tubular epithelial cell line HK-2. With the use of DNA microarrays the transcriptomes of HK-2 cells cultured in mono- and coculture were compared. HK-2 cells in coculture exhibited a differential expression of 99 genes involved in pathways such as extracellular matrix (e.g., lysyl oxidase), cell-cell communication (e.g., IL-6 and IL-1 beta), and transport (e.g., GLUT3 and lipocalin 2). HK-2 cells also exhibited an enhanced paracellular gating function in coculture, which was dependent on HMEC-1-derived extracellular matrix. We identified a number of HMEC-1-enriched genes that are potential regulators of epithelial cell function such as extracellular matrix proteins (e.g., collagen I, III, IV, and V, laminin-alpha IV) and cytokines/growth factors (e.g., hepatocyte growth factor, endothelin-1, VEGF-C). This study demonstrates a complex network of communication between microvascular endothelial cells and proximal tubular epithelial cells that ultimately affects proximal tubular cell function. This coculture model and the data described will be important in the further elucidation of microvascular endothelial and proximal tubular epithelial cross talk mechanisms.
Authors:
Sonia Aydin; Sara Signorelli; Thomas Lechleitner; Michael Joannidis; Clara Pleban; Paul Perco; Walter Pfaller; Paul Jennings
Related Documents :
6775349 - Enhancement of prostaglandin synthesizing activity by the treatment with sodium n-butyr...
3465969 - Leukotriene c4 and d4 contract rat glomerular mesangial cells.
22673369 - High-purity enrichment of functional cardiovascular cells from human ips cells.
1349779 - An ultrastructural study of proliferative nephritis induced experimentally by a monoclo...
7642529 - Tissue-specific enhancer-promoter interactions regulate high level constitutive express...
11286009 - The contribution of mesangial cell proliferation to progressive glomerular injury.
16740359 - Retinoic acid attenuates promyelocytic leukemia protein-induced cell death in breast ca...
372499 - Glial fibrillary acidic protein in ependymomas and other brain tumors. distribution, di...
9557679 - The transmembrane domains of sindbis virus envelope glycoproteins induce cell death.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-05
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  294     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-15     Completed Date:  2008-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C543-54     Citation Subset:  IM    
Affiliation:
Division of Physiology, Department of Physiology and Medical Physics, Innsbruck Medical University, Innsbruck, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Capillaries / metabolism,  ultrastructure
Cell Communication / genetics*
Cell Line
Cell Line, Transformed
Cell Membrane Permeability / genetics
Coculture Techniques
Endothelium, Vascular / cytology,  metabolism*
Epithelial Cells / cytology,  metabolism*
Extracellular Matrix Proteins / genetics
Gene Expression Regulation / genetics
Humans
Intercellular Signaling Peptides and Proteins / genetics
Kidney / blood supply,  metabolism
Kidney Tubules, Proximal / cytology,  metabolism*
Oligonucleotide Array Sequence Analysis
Signal Transduction / genetics
Transcription, Genetic / genetics*
Up-Regulation / genetics
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Intercellular Signaling Peptides and Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bistable MAP kinase activity: a plausible mechanism contributing to maintenance of late long-term po...
Next Document:  Opposing effects of coupled and uncoupled NOS activity on the Na+-K+ pump in cardiac myocytes.