Document Detail


Influence of metabolic fuel on the 13C/12C ratio of breath CO2.
MedLine Citation:
PMID:  6441607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Natural differences in 13C/12C ratios of various metabolic fuels can produce systematic changes in the 13C/12C ratio of breath CO2, and therefore introduce errors into 13CO2 breath tests. To gain insight into the potential problem, we compared 13C/12C ratios of plasma macronutrients to those of breath CO2 under conditions that should alter the percentages of carbohydrate and lipid being oxidized. In rats, 48 h of starvation decreased the 13C/12C ratio of breath CO2 by 3.5%. At this time the 13C/12C ratio of breath CO2 was very similar to that of plasma lipids. In humans, 30 min of heavy exercise increased the breath 13CO2/12CO2 ratio by 1.3%. These changes in breath 13C/12C ratios could be predicted from 13C/12C ratios of plasma macronutrients and the percentage of carbon dioxide derived from each macronutrient, but only when compared within the same populations. For example, the 13C/12C ratios of plasma macronutrients of residents of Chicago, Illinois (USA) and Tokyo (Japan) differed by 1-3%. An empirical correction of 13CO2 breath test data is recommended when breath tests are run under conditions that will change metabolic fuel utilization.
Authors:
D A Schoeller; C Brown; K Nakamura; A Nakagawa; R S Mazzeo; G A Brooks; T F Budinger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomedical mass spectrometry     Volume:  11     ISSN:  0306-042X     ISO Abbreviation:  Biomed. Mass Spectrom.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1985-04-11     Completed Date:  1985-04-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0430246     Medline TA:  Biomed Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  557-61     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Blood Glucose / analysis
Blood Proteins / analysis
Breath Tests*
Carbon Dioxide / analysis*
Carbon Isotopes / analysis
Energy Metabolism*
Humans
Lipids / blood
Male
Physical Exertion
Pulmonary Gas Exchange*
Rats
Starvation / metabolism
Grant Support
ID/Acronym/Agency:
AM-19577/AM/NIADDK NIH HHS; AM-26678/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Blood Proteins; 0/Carbon Isotopes; 0/Lipids; 124-38-9/Carbon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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