| Influence of mebendazole on insulin secretion from isolated rat islets. | |
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MedLine Citation:
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PMID: 3519938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The antihelminthic drug mebendazole (MEB) has been reported to elevate circulating insulin levels in vivo. By using isolated perifused rat islets we tested the acute effects of this drug directly on the beta cell in the absence or presence of exogenous glucose. In the absence of glucose, MEB (40 micrograms/ml) caused a delayed but sustained increase in insulin output from the islet. Release induced by this concentration of MEB was not influenced by either forskolin (10 microM), a compound that increases islet cyclic AMP levels and potentiates glucose-induced secretion, or the absence of extracellular calcium. In the presence of a submaximal stimulatory glucose concentration (8 mM), release was dramatically potentiated by the further addition of 10 to 40 micrograms/ml of MEB. The potentiating effect of the drug on glucose-induced release was readily reversible and abolished by the omission of extracellular calcium or the addition of 10 mM mannoheptulose, a compound that blocks glucose phosphorylation. Despite its positive effects on hormone secretion, MEB did not affect glucose metabolism. MEB may prove useful for investigating the factors that regulate insulin secretion. |
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Authors:
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W S Zawalich; V Diaz; S Cote |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 237 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 1986 Jun |
Date Detail:
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Created Date: 1986-07-16 Completed Date: 1986-07-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 826-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Glucose / metabolism, pharmacology Insulin / secretion* Islets of Langerhans / drug effects*, secretion Male Mebendazole / pharmacology* Microtubules / drug effects Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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AM00944/AM/NIADDK NIH HHS; AM34381/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 31431-39-7/Mebendazole; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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