| Influence of the maize silage to grass silage ratio and feed particle size of rations for ruminants on the community structure of ruminal Firmicutes in vitro. | |
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MedLine Citation:
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PMID: 20722875 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: To investigate the effect of the forage source and feed particle size (FPS) in ruminant rations on the composition of the ruminal Firmicutes community in vitro. METHODS AND RESULTS: Three diets, varying in maize silage to grass silage ratio and FPS, were incubated in a rumen simulation system. Microbial samples were taken from the liquid fermenter effluents. Microbial community analysis was performed by 16S rRNA-based techniques. Clostridia-specific single-strand conformation polymorphism profiles revealed changes of the community structure in dependence on both factors tested. The coarse grass silage-containing diets seemed to enhance the occurrence of different Roseburia species. As detected by real-time quantitative PCR, Ruminococcus albus showed a higher abundance with decreasing FPS. A slightly lower proportion of Bacilli was found with increasing grass silage to maize silage ratio by fluorescence in situ hybridization (FISH). In contrast, a slightly higher proportion of bacterial species belonging to the Clostridium-clusters XIV a and b was detected by FISH with increasing grass silage contents in the diet. CONCLUSIONS: The ruminal Firmicutes community is affected by the choice of the forage source and FPS. SIGNIFICANCE AND IMPACT OF THE STUDY: This study supplies fundamental knowledge about the response of ruminal microbial communities to changing diets. Moreover, the data suggest a standardization of grinding of feeds for in vitro studies to facilitate the comparison of results of different laboratories. |
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Authors:
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M Witzig; J Boguhn; S Kleinsteuber; I Fetzer; M Rodehutscord |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1998-2010 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
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Universität Hohenheim, Institut für Tierernährung, Stuttgart, Germany. inst450@uni-hohenheim.de |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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