| Influence of macroporous gold support and its functionalization on lactate oxidase-based biosensors response. | |
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MedLine Citation:
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PMID: 22608456 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A general bioanalytical platform for biosensor applications was developed based on three-dimensional ordered macroporous (3DOM) gold film modified electrodes using lactate oxidase (LOx) as a case study, within the framework of developing approaches of broad applicability. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold electrode up to 18 times higher than that of bare flat gold electrodes. These new electrochemical transducers were characterized by using Field Emission Scanning Electron Microscopy (FE-SEM), Atomic Force Microscopy (AFM) and the X-ray diffraction (XRD). The biosensor was developed by immobilization of lactate oxidase (LOx), on a 3DOM gold electrode modified with a self-assembled monolayer of dithiobis-N-succinimidyl propionate (DTSP). The resulting lactate oxidase biosensor was characterized by electrochemical impedance spectroscopy (EIS). The 3DOM gold electrode not only provides a good biocompatible microenvironment but also promotes the increase of conductivity and stability. Thus, the developed lactate oxidase bioanalytical platforms showed higher mediated bioelectrocatalytic activity compared to others previously described based on polycrystalline gold transducers. The response to varying lactate concentrations has been obtained in the presence of hydroxymethylferrocene as redox mediator in solution. Under these conditions, the bioanalytical platform response for DTSP covalently bound enzyme was improved with respect to that obtained in absence of DTSP. |
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Authors:
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M Gamero; M Sosna; F Pariente; E Lorenzo; P N Bartlett; C Alonso |
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Publication Detail:
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Type: Journal Article Date: 2012-04-04 |
Journal Detail:
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Title: Talanta Volume: 94 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 328-34 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Applied Physical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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