| Influence of lysine on urea cycle activity and orotate formation in the isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 3924076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There was a reversible inhibition of urea formation in the perfused rat liver caused by 2.25-27 mM lysine acting with a Ki of 10.8 mM in competition with ornithine. Urea formation in the presence of inhibitory concentrations of lysine ranged between 2.3 and 2.9 mumol X min-1 X (g, liver wet)-1 after addition of 1 mM of citrulline, argininosuccinate or arginine, whereas it amounted to 0.5 mumol X min-1 X (g, liver wet)-1 after addition of ornithine, showing that lysine inhibited the urea cycle between ornithine and citrulline. There was a rise of basal orotate formation of 0.03 +/- 0.02 mumol X h-1 X (g, liver wet)-1 towards a maximum of 0.6 +/- 0.04 mumol X h-1 X (g, liver wet)-1 after addition of 13.5 mM lysine, provided orotate utilization was blocked with allopurinol. Maximal rates of orotate formation were reached when ammonium concentrations exceeded 1 mM. We conclude that an inhibition of urea synthesis and a rise of orotate formation are caused by lysine in the isolated liver in vitro at rates observed in vivo. Hence, these metabolic alterations observed in the whole animal are most probably due to changes of liver metabolism. |
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Authors:
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M de Vrese; C A Barth |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biological chemistry Hoppe-Seyler Volume: 366 ISSN: 0177-3593 ISO Abbreviation: Biol. Chem. Hoppe-Seyler Publication Date: 1985 May |
Date Detail:
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Created Date: 1985-08-22 Completed Date: 1985-08-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8503054 Medline TA: Biol Chem Hoppe Seyler Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 455-61 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonia
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pharmacology Animals Kinetics Liver / drug effects, metabolism* Lysine / pharmacology* Male Ornithine / pharmacology Orotic Acid / biosynthesis* Perfusion Rats Rats, Inbred Strains Urea / metabolism* |
| Chemical | |
Reg. No./Substance:
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56-87-1/Lysine; 57-13-6/Urea; 65-86-1/Orotic Acid; 7006-33-9/Ornithine; 7664-41-7/Ammonia |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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