Document Detail


Influence of long-chain polyunsaturated fatty acid formula feeds on vitamin E status in preterm infants.
MedLine Citation:
PMID:  14639802     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been recommended to supplement formulas for preterm infants with n-3 and n-6 long-chain polyunsaturated fatty acids (LCP) to improve growth, visual acuity, and neurodevelopmental performance. However, large amounts of LCP may increase lipid peroxidation and oxidative stress in preterm infants. We investigated if, under high supplementation of natural tocopherols, LCP addition to formula can be performed safely without causing tocopherol depletion in cell membranes. Thirty-one healthy preterm infants with gestational ages from 28 to 32 weeks were evaluated in a prospective, randomized study from birth to day 42. Nine infants received an n-3 and n-6 LCP-enriched formula (A), eleven infants a standard formula (B), and eleven infants breast milk (control group). Alpha- and gamma-tocopherol extracts were added to both formulas, amounting to five times the value in breast milk (2.3 mg/dL in both formulas versus 0.45 mg/dL in breast milk). Erythrocyte arachidonic acid (AA) and docosahexaenoic acid (DHA) in the phosphatidylethanolamine fraction were similar in the three groups over the study period, whereas a significant reduction of erythrocyte AA and DHA could be detected in the phosphatidylcholine fraction in all three groups from day 14 onwards, when compared to respective cord blood values, with lowest values in the standard formula group. Amazingly, levels of alpha- and gamma-tocopherol were higher in plasma, erythrocytes, platelets, monocytes, and polymorphonuclear leukocytes with LCP supplementation as compared to standard formula and breast milk from day 7 onwards, whereas in buccal mucosal cells, this was not the case until day 42. Gammatocopherol uptake in the LCP-supplemented group was also significantly higher in all cell fractions studied from day 7 onwards. We therefore hypothesize that the LCP supplementation used in formula A improves tocopherol solubility and stability in biological membranes. Under high-dose vitamin E addition to n-3 and n-6 LCP-supplemented formula, no evidence for tocopherol depletion and furthermore, high accumulation of tocopherols, can be detected in healthy preterm infants.
Authors:
Daisy E Kaempf-Rotzoll; Gerald Hellstern; Otwin Linderkamp
Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition     Volume:  73     ISSN:  0300-9831     ISO Abbreviation:  Int J Vitam Nutr Res     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-26     Completed Date:  2004-04-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1273304     Medline TA:  Int J Vitam Nutr Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  377-87     Citation Subset:  IM    
Affiliation:
Graduate School of Pharmaceutical Sciences, Dept. of Health Chemistry, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113-0033 Tokyo, Japan. rotzolld@mol.f.u-tokyo.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / administration & dosage*,  metabolism
Arachidonic Acids / metabolism
Biological Markers / blood
Body Weight / physiology
Docosahexaenoic Acids / metabolism
Erythrocyte Membrane / metabolism
Fatty Acids, Unsaturated / administration & dosage*
Female
Fetal Blood / chemistry,  metabolism
Humans
Infant Formula / administration & dosage*
Infant Nutritional Physiological Phenomena / physiology
Infant Welfare
Infant, Newborn
Infant, Premature
Male
Milk, Human / chemistry,  metabolism
Mouth Mucosa / metabolism
Prospective Studies
Vitamin E / administration & dosage*
alpha-Tocopherol / metabolism
gamma-Tocopherol / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Arachidonic Acids; 0/Biological Markers; 0/Fatty Acids, Unsaturated; 0/gamma-Tocopherol; 1406-18-4/Vitamin E; 25167-62-8/Docosahexaenoic Acids; 59-02-9/alpha-Tocopherol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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