| Influence of the intestinal anticoagulant in the feeding performance of triatomine bugs (Hemiptera; Reduviidae). | |
| | |
MedLine Citation:
|
PMID: 21447340 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Triatomines are hematophagous insects in all post-embryonic life stages. They are vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Their vectorial ability is influenced by their feeding performance, which varies greatly among species. Recent work showed that inhibition of the coagulation process in the anterior midgut (crop) environment considerably influences the blood meal size. In this work, we performed a comparative study of the level of anticoagulant activity in the saliva and crop contents of three triatomine species - Triatoma infestans,Triatoma brasiliensis and Rhodnius prolixus - and correlated this with their feeding performance on live hosts. Moreover, the feeding parameters on a large diameter vessel influenced by the crop anticoagulants were evaluated in detail. The anticoagulant activity was significantly higher in the crop contents than in salivary glands, varying from 1.6-fold higher for R. prolixus to 70-fold higher for T. brasiliensis. Among the species, T. brasiliensis had the lowest crop anticoagulant activity, the lowest concentration of thrombin inhibitor, and took the longest to feed. Triatoma brasiliensis nymphs that had their intestinal anticoagulant (brasiliensin) knocked down by RNA interference had the lowest capacity to maintain cibarial pump frequency at higher levels throughout the feeding process and consequently a lower ingestion rate (mg/min), even when fed under favorable conditions (large diameter vessel). However, the feeding difficulty for brasiliensin knockdown T. brasiliensis nymphs was reversed by treating the host mice with heparin (a potent systemic anticoagulant) before blood feeding. The results indicate that crop anticoagulant activity influences modulation of the blood-pumping frequency to the intestine and significantly affects the feeding efficiency of triatomine spp. on live hosts. |
| | |
Authors:
|
Rafaela M M Paim; Ricardo N Araújo; Adriana C Soares; Lucas C Dhom Lemos; Aparecida S Tanaka; Nelder F Gontijo; Michael J Lehane; Marcos H Pereira |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-26 |
Journal Detail:
|
Title: International journal for parasitology Volume: - ISSN: 1879-0135 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0314024 Medline TA: Int J Parasitol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
|
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Bloco I4, Sala 177, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of a polypeptide containing Tudor and staphyloccocal nuclease-like domains as the seq...
Next Document: Outcrossing and crossbreeding recovers deteriorated traits in laboratory cultured Steinernema carpoc...