Document Detail

Influence of hyaluronic acid or phorbol 12-myristate 13-acetate on the migration capacity of a murine lymphoma cell line (Eb) and its metastatic variant (ESb).
MedLine Citation:
PMID:  9459209     Owner:  NLM     Status:  MEDLINE    
The in vitro migration of two murine T cell lymphoma cell lines (Eb and ESb) was studied employing a three-dimensional collagen matrix and time-lapse video recording. In the highly metastatic cell line ESb, which had a low spontaneous locomoting activity, migration could clearly be stimulated by hyaluronic acid (HA) whereas only a small increase was found after incubation with phorbol myristate acetate (PMA). The observed stimulation could be attributed to an increase in recruitment of locomoting cells and not to changes in migration parameters of motile individual cells such as percentage of time locomoting, velocity or distance migrated. Incubation of the low metastatic cell line Eb with HA led to a decrease in migration but blocking of CD44, the principle ligand for HA, by preincubation with an anti-CD44 mAb (KM114), followed by HA exposure increased the locomoting activity significantly. The effect was based on both an increase in recruitment as well as in all migration parameters regarding motile individual Eb cells.
B S Kubens; G Nikolai; K S Zänker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  118     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-02-24     Completed Date:  1998-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  189-200     Citation Subset:  IM    
Institute of Immunology, University Witten/Herdecke, Witten, Germany.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigens, CD44 / metabolism
Cell Movement / drug effects
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Hyaluronic Acid / pharmacology*
Lymphoma, T-Cell / pathology*
Neoplasm Metastasis*
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD44; 0/Ligands; 16561-29-8/Tetradecanoylphorbol Acetate; 9004-61-9/Hyaluronic Acid

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