Document Detail


Influence of hyaluronic acid or phorbol 12-myristate 13-acetate on the migration capacity of a murine lymphoma cell line (Eb) and its metastatic variant (ESb).
MedLine Citation:
PMID:  9459209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro migration of two murine T cell lymphoma cell lines (Eb and ESb) was studied employing a three-dimensional collagen matrix and time-lapse video recording. In the highly metastatic cell line ESb, which had a low spontaneous locomoting activity, migration could clearly be stimulated by hyaluronic acid (HA) whereas only a small increase was found after incubation with phorbol myristate acetate (PMA). The observed stimulation could be attributed to an increase in recruitment of locomoting cells and not to changes in migration parameters of motile individual cells such as percentage of time locomoting, velocity or distance migrated. Incubation of the low metastatic cell line Eb with HA led to a decrease in migration but blocking of CD44, the principle ligand for HA, by preincubation with an anti-CD44 mAb (KM114), followed by HA exposure increased the locomoting activity significantly. The effect was based on both an increase in recruitment as well as in all migration parameters regarding motile individual Eb cells.
Authors:
B S Kubens; G Nikolai; K S Zänker
Related Documents :
1850369 - A possible role for dif-2 in the formation of stalk cells during dictyostelium developm...
11730019 - Automated real-time measurement of chemotactic cell motility.
25486409 - Autophagosome-mediated egfr down-regulation induced by the ck2 inhibitor enhances the e...
21428939 - Exosome target cell selection and the importance of exosomal tetraspanins: a hypothesis.
18205049 - Microfluidic switching system for analyzing chemotaxis responses of wortmannin-inhibite...
20807799 - The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers p...
1165439 - Calcitonin production by rat thyroid tumours.
15243649 - Flow cytometry based techniques to study testicular acidophilic granulocytes from the p...
10379899 - Molecular characterization of the follicle defects in the growth differentiation factor...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  118     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-02-24     Completed Date:  1998-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  189-200     Citation Subset:  IM    
Affiliation:
Institute of Immunology, University Witten/Herdecke, Witten, Germany. bks@uwh.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antigens, CD44 / metabolism
Cell Movement / drug effects
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Hyaluronic Acid / pharmacology*
Ligands
Lymphoma, T-Cell / pathology*
Mice
Neoplasm Metastasis*
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD44; 0/Ligands; 16561-29-8/Tetradecanoylphorbol Acetate; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Migration and gap junctional intercellular communication determine the metastatic phenotype of human...
Next Document:  Studies on the cytostatic and cytotoxic effects and mode of action of 8-nitro-7-hydroxycoumarin.