| Influence of high hydrostatic pressure on the proteolysis of beta-lactoglobulin A by trypsin. | |
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MedLine Citation:
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PMID: 16433971 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work describes the effect of the hydrolysis time and pressure (0.1-400 MPa) on the proteolysis of beta-lactoglobulin A (beta-lg A) with trypsin, either conducting hydrolysis of beta-lg under pressure or hydrolysing beta-lg that was previously pressure treated. Pressurisation, before or during enzyme treatments, enhanced tryptic hydrolysis of beta-lg. Trypsin degraded pressure-modified beta-lg and pressure-induced beta-lg aggregates, favouring proteolysis to the intermediate degradation products: (Val(15)-Arg(40)), (Val(41)-Lys(69))S-S(Leu(149)-Ile(162)) and (Val(41)-Lys(70))S-S(Leu(149)-Ile(162)). These were further cleaved at the later stages of proteolysis to yield: (Val(15)-Tyr(20)), (Ser(21)-Arg(40)), (Val(41)-Tyr(60)), (Trp(61)-Lys(69))S-S(Leu(149)-Ile(162)) and (Trp(61)-Lys(70))S-S(Leu(149)-Ile(162)). Particularly, in the tryptic hydrolysates of pre-pressurized beta-lg, two other fragments linked by disulphide bonds: (Lys(101)-Arg(124))S-S(Leu(149)-Ile(162)) and (Tyr(102)-Arg(124))S-S(Leu(149)-Ile(162)), were found. These corresponded to rearrangement products induced by SH/SS exchange between the free thiol group of Cys(121) and Cys(160), that normally forms the disulphide bond Cys(66)-Cys(160). In the light of these results, structural modifications of beta-lg under high pressure are discussed. |
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Authors:
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Rosa Chicón; Josefina Belloque; Isidra Recio; Rosina López-Fandiño |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of dairy research Volume: 73 ISSN: 0022-0299 ISO Abbreviation: J. Dairy Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-25 Completed Date: 2006-05-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: England |
Other Details:
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Languages: eng Pagination: 121-8 Citation Subset: IM |
Affiliation:
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Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Hydrolysis Hydrostatic Pressure* Lactoglobulins / metabolism* Milk / enzymology*, metabolism Time Factors Trypsin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Lactoglobulins; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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