Document Detail

Influence of heart failure on nucleolar organization and protein expression in human hearts.
MedLine Citation:
PMID:  22244875     Owner:  NLM     Status:  Publisher    
We investigate for the first time the influence of heart failure (HF) on nucleolar organization and proteins in patients with ischemic (ICM) or dilated cardiomyopathy (DCM). A total of 71 human hearts from ICM (n=38) and DCM (n=27) patients, undergoing heart transplantation and control donors (n=6), were analysed by western-blotting, RT-PCR and cell biology methods. When we compared protein levels according to HF etiology, nucleolin was increased in both ICM (117%, p<0.05) and DCM (141%, p<0.01). Moreover, mRNA expression were also upregulated in ICM (1.46-fold, p<0.05) and DCM (1.70-fold, p<0.05. Immunofluorescence studies showed that the highest intensity of nucleolin was into nucleolus (p<0.0001), and it was increased in pathological hearts (p<0.0001). Ultrastructure analysis by electron microscopy showed an increase in the nucleus and nucleolus size in ICM (17%, p<0.05 and 131%, p<0.001) and DCM (56%, p<0.01 and 69%, p<0.01). Nucleolar organization was influenced by HF irrespective of etiology, increasing fibrillar centers (p<0.001), perinucleolar chromatin (p<0.01) and dense fibrillar components (p<0.01). Finally, left ventricular function parameters were related with nucleolin levels in ischemic hearts (p<0.0001). The present study demonstrates that HF influences on morphology and organization of nucleolar components, revealing changes in the expression and in the levels of nucleolin protein.
Esther Roselló-Lletí; Miguel Rivera; Raquel Cortés; Inmaculada Azorín; Rafael Sirera; Luis Martínez-Dolz; Leif Hove; Juan Cinca; Francisca Lago; José R González-Juanatey; Antonio Salvador; Manuel Portolés
Related Documents :
25416385 - Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction.
25287895 - Effect of chronic heart rate reduction by i f current inhibitor ivabradine on left vent...
25447345 - Effects of left ventricular assist device support on biomarkers of cardiovascular stres...
2889585 - An experimental contribution to the treatment of acute myocardial infarction.
11878575 - Evaluation of left ventricular function in asymptomatic children about to undergo anthr...
21685265 - Prostanoids suppress the coronary vasoconstrictor influence of endothelin after myocard...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-8
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  -     ISSN:  1090-2104     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Cardiocirculatory Unit, Research Center, Hospital Universitario La Fe, Valencia, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Increase of L-type Ca(2+) current by protease-activated receptor 2 activation contributes to augment...
Next Document:  Extracellular acidosis accelerates bone resorption by enhancing osteoclast survival, adhesion, and m...