Document Detail


Influence of the growth substrate on ester-linked phospho- and glycolipid fatty acids of PAH-degrading Mycobacterium sp. LB501T.
MedLine Citation:
PMID:  12871234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influences of poorly water-soluble anthracene on ester-linked phospholipid fatty acid (PLFA) and glycolipid fatty acid (GLFA) profiles of Mycobacterium sp. LB501T were studied. Bacteria were cultivated on either anthracene or glucose (one culture with successively amended small doses of this substrate and one with excess concentrations) to distinguish between influences of the chemical structure and the bioavailability of the growth substrate. Results revealed that GLFA and PLFA profiles of M. sp. LB501T depended on the availability and the structure of the carbon source. Fatty acid profiles obtained with anthracene differed from those obtained with excess glucose. They were interpreted as a specific adaptation to this poorly bioavailable polycyclic aromatic hydrocarbon (PAH). In contrast, profiles obtained with low glucose concentrations showed clear signs of starvation stress. Stable carbon isotopic ratios (delta13C) of GLFA and PLFA of M. sp. LB501T were analysed to characterize the 13C-fractionation during the biosynthesis of individual fatty acids and to evaluate their value as markers for substrate usage. Although the delta13C values of PLFA and GLFA showed differential isotope fractionation during anthracene- and glucose-degradation, they were sufficiently distinct to be used as signatures of bacterial substrate usage.
Authors:
Lukas Y Wick; Oliver Pelz; Stefano M Bernasconi; Nils Andersen; Hauke Harms
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental microbiology     Volume:  5     ISSN:  1462-2912     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-21     Completed Date:  2003-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  672-80     Citation Subset:  IM    
Affiliation:
Swiss Federal Institute of Technology Lausanne (EPFL), ENAC/ISTE-Laboratory of Soil Science, CH-1015 Lausanne, Switzerland. lukas.wick@epfl.ch
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MeSH Terms
Descriptor/Qualifier:
Anthracenes / metabolism*
Biodegradation, Environmental
Carbon Isotopes / analysis
Culture Media
Fatty Acids / metabolism*
Glucose / metabolism
Glycolipids / metabolism*
Mycobacterium / growth & development,  metabolism*
Phospholipids / metabolism*
Polycyclic Hydrocarbons, Aromatic / metabolism*
Chemical
Reg. No./Substance:
0/Anthracenes; 0/Carbon Isotopes; 0/Culture Media; 0/Fatty Acids; 0/Glycolipids; 0/Phospholipids; 0/Polycyclic Hydrocarbons, Aromatic; 120-12-7/anthracene; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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