Document Detail


Influence of glygerol on ATP and 2,3-DPG levels of human erythrocytes.
MedLine Citation:
PMID:  462914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycerolization of freshly collected human erythrocytes leads to a reduction in ATP levels which return to their original values following deglycerolization. The reduction in ATP levels is largely prevented by pyruvate but not by inosine and glucose, singly or in combination. This implies that the ATP lesion associated with glycerolization reflects mainly a decrease in the NAD/NADH ratio and accordingly a reduced glyceraldehyde-3-phosphate dehydrogenase activity. 2,3-DPG levels were not influenced by glycerolization and deglycerolization. Erythrocytes depleted of ATP in the presence of glycerol can have their ATP levels repleted by either pyruvate or inosine, but require the presence of both compounds for maintenance during a post-rejuvenation incubation period. This indicates that the prolonged presence of glycerol influences both the NAD/NADH ratio and the carbon-containing intermediates of glycolysis. Glycerol did not influence ATP repletion of erythrocytes stored at 4 degrees C for 20 days by inosine in combination with pyruvate, but substantially decreased the repletion of their 2,3-DPG levels.
Authors:
G Moroff; H T Meryman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Vox sanguinis     Volume:  36     ISSN:  0042-9007     ISO Abbreviation:  Vox Sang.     Publication Date:  1979  
Date Detail:
Created Date:  1979-09-25     Completed Date:  1979-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  244-51     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / blood*
Diphosphoglyceric Acids / blood*
Erythrocytes / metabolism*
Glucose / metabolism
Glycerol / pharmacology*
Humans
Inosine / metabolism
Pyruvates / metabolism
Chemical
Reg. No./Substance:
0/Diphosphoglyceric Acids; 0/Pyruvates; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 56-81-5/Glycerol; 58-63-9/Inosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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