| Influence of glucose and other substrates on electric field and polyethylene glycol-mediated transformation of intact yeast cells. | |
| | |
MedLine Citation:
|
PMID: 7926665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A prepulse incubation with 2% glucose (mannose, fructose) strongly inhibited electrotransformation of intact yeast cells. This inhibitory effect was not due to alterations of cell viability or to cell membrane electropermeabilization, and was not affected by the solution buffer properties of preincubation and pulsing media. The electrotransformation efficiency was not modified by non-metabolized substances, such as sorbitol or alose. The glucose inhibition of electrotransformation was fast, occurring after only a 3-5-min preincubation. The postpulse incubation with substrate also decreased transformation efficiency, but was pH-dependent. We observed a similar, strong inhibitory effect of glucose when cells were transformed chemically. A pH dependence of yeast electrotransformation was established. |
| | |
Authors:
|
V Ganeva; B Galutzov; J Teissie |
Related Documents
:
|
17309505 - Relationship between growth and ph gradients of individual cells of debaryomyces hansen... 5847805 - Biotin transport and accumulation by cells of lactobacillus plantarum. i. general prope... 6755125 - Rat adipocyte utilization of different substrates: effects of cell size and the control... 11911485 - Betulinic acid sensitization of low ph adapted human melanoma cells to hyperthermia. 21098705 - P21cip-1/waf-1 induction is required to inhibit prostate cancer growth elicited by defi... 2358985 - X-ray microanalysis of rotavirus-infected mouse intestine: a new concept of diarrhoeal ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 121 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 1994 Aug |
Date Detail:
|
Created Date: 1994-11-09 Completed Date: 1994-11-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 159-64 Citation Subset: IM |
Affiliation:
|
Department of Biophysics and Radiobiology, Faculty of Biology, Sofia University, Bulgaria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
analysis Electricity Glucose / pharmacology* Hydrogen-Ion Concentration Polyethylene Glycols / pharmacology* Saccharomyces cerevisiae / cytology* Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/Polyethylene Glycols; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cloning and expression of the Bacteroides fragilis YCH46 neuraminidase gene in Escherichia coli and ...
Next Document: Properties of a thermostable 4Fe-ferredoxin from the hyperthermophilic bacterium Thermotoga maritima...